The present study was carried out for identification of the phytochemicals present in the Delonix regia leaves and also evaluates the total phenols, total flavonoids and antioxidant activity of the leaf extract. Total phenols were carried out by Folin Ciocalteu method and the phenolic content was 16.00 mg/100 g of gallic acid equivalent (GE). Antioxidant activity was evaluated by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) method and the leaves of D. regia showed 10.73 mg/100 g of ascorbic acid equivalent antioxidant capacity (AEAC). The gas chromatograph and mass spectrometer (GC-MS) study was also carried out and it showed the presence of phytochemicals like phytol (RT: 15.49), Cumarin 7, 8-dihyddro-7-hydroxy-6-methoxy-8-oxo (RT: 15.92), Squalene (RT: 25.41) and Vitamine (RT: 29.97).
Key words: Total phenols, total flavonoids, antioxidant activity, 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), gas chromatograph and mass spectrometer (GC-MS), delonix regia.
GE, Gallic acid equivalent; DPPH, 2,2-diphenyl-1-picrylhydrazyl; AEAC, acid equivalent antioxidant capacity; GC-MS, gas chromatograph and mass spectrometer; UV,ultraviolet; NIST, National Institute Standard and Technology; O.D, optical density.
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