African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

Trans-resveratrol as phenolic indicator of somatic embryogenesis induction in cotton (Gossypium hirsutum L.) cell suspensions

Kouakou Tanoh Hilaire1, 2*, Koné Mongomaké2, Koné Daouda3, Kouadio Yatty Justin2, Amani N’guessan Georges4, Téguo Waffo Pierre1, Décendit Alain1 and Mérillon Jean-Michel1
1Laboratoire de Mycologie et Biotechnologie Végétale, UFR des Sciences Pharmaceutiques, GESVAB, EA 3675, Université de bordeaux 2, 146 rue Léo-Saignat F-33076 Bordeaux cedex, France. 2Laboratoire de Biologie et d’Amélioration des Productions végétales, UFR Sciences de la Nature, Université d’Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire. 3Laboratoire de Physiologie Végétale, UFR Biosciences, Université de Cocody, 22 BP 582 Abidjan 22, Côte d’Ivoire. 4Laboratoire de Technologie Alimentaire, UFR des Sciences et Technologie des Aliments, Université d’Abobo-Adjamé, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Email: [email protected]

  •  Accepted: 03 January 2008
  •  Published: 31 January 2008


Trans-resveratrol is a phytoalexin which was found in some grapes and in many other plants. This phenolic compound has gained much attention, as it was indicated to be associated with fungus and disease resistance. Also, it has been shown that phenols compounds could play an important role in somatic embryogenesis induction of various plants. In cotton, embryogenic structures take place in cell suspension cultures. The need of biochemical indicators for the early identification of cells capable to undergo embryogenic structures formation has always been a major concern of researchers. Trans-resveratrol was found only in embryogenic cell suspensions of Coker 312. This phenolic compound started at the first subculture (2.44 µg/g dw) and the maximum level was reached at the third subculture (7.2 µg/g dw) with an increase of nearly 295%. This study showed a correlation between trans-resveratrol synthesis in cotton cell and embryogenic structures induction. trans-resveratrol may be a phenolic indicator for is induction of cotton somatic embryogenesis.


Key words: Trans-resveratrol, suspension culture, embryogenic structures, cotton, Gossypium hirsutum L.