African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 408

Short Communication

Comparative study on the determination of assay for laccase of Trametes sp.

Airong Li, Yue Zhu, Liang Xu, Wenqing Zhu and Xingjun Tian*
School of Life Science, Nanjing University, Nanjing 210093, P. R. China.
Email: [email protected]

  •  Accepted: 14 August 2008
  •  Published: 31 August 2008

Abstract

The aim of this work was to determine the sensitivity among compounds in common use for detecting laccase activity. In this study, three assay procedures to measure laccase activity of Trametes sp. were performed in Kirk’s basal salts mediums of three dyes. In the assay methods, three substrates were employed, which were 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), ο-dianisidine and guaiacol. The results indicate that laccase activity used ABTS as a substrate was significantly higher than the other two. Therefore, the ABTS method is recommended while detecting laccase activity.

Key words: ABTS, ο-dianisidine, guaiacol, laccase activity, Trametes sp.