African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 425

Full Length Research Paper

Evaluation of different matrices for production of alkaline protease from Bacillus subtilis –K 30 by entrapment technique

K. Suresh Babu Naidu1*, K. Lakshmi Devi2 and K. Jamila Adam1
1Department of Biomedical and Clinical Technology, Durban University of Technology, Durban-4000, South Africa. 2Faculty of Biochemistry, SriKrishna Devaraya University, Anantapur-515 003, Andhra Pradesh, India.
Email: [email protected]

  •  Accepted: 08 July 2011
  •  Published: 31 July 2011


The present investigation evaluates the suitability of different matrices such as calcium alginate, polyacrylamide, agar-agar and gelatin for production of alkaline protease from Bacillus subtilis-K 30 using immobilization approach. Calcium alginate was found to be an effective and suitable matrix for higher alkaline protease productivity compared to other matrices studied. All the matrices were selected for repeated batch fermentation. The average specific volumetric productivities with calcium alginate was 14.83 U/ml/h which is 42.83% higher production over the convention free cell fermentation. Similarly, the specific volumetric productivity by repeated batch fermentation was 12.29 U/ml/h with polyacrylamide, 12.22 U/ml/h with agar-agar and 12.09 U/ml/h with gelatin. From the results, it is concluded that the immobilized cells of B. subtilis K-30 in calcium alginate are more efficient for the production of alkaline protease with repeated batch fermentation.


Key words: Bacillus subtilis K-30, Alkaline protease, immobilized cells, repeated batch fermentation, matrices.


DCW, Dry cell weight; TEMED, tetra methyl ethylene diamine.