African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 408

Full Length Research Paper

The apoptotic and cytotoxic effects of Polygonum avicular extract on Hela-S cervical cancer cell line

Rahmati Mohammad1*, Bannazadeh Hossein2, Fazli Davood3, Tahmasebzadeh Farnaz3, Farrokhi Ali4 and Rasmi Yusef5
1Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 2Department of Clinical microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. 3 Department of Basic Sciences, Payame Noor University, PO BOX 19395-3697 Tehran, Iran. 4Royan Institute, Tehran, Iran. 5Department of Clinical Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Email: [email protected]

  •  Accepted: 22 November 2011
  •  Published: 31 December 2011

Abstract

Cervical cancer is the second main cause of cancer leading death in women. Therefore, targeting it, preferably with natural compounds derived from medicinal plant such as Polygonum avicular as an herbal extract have astringent properties. Herbal medicines in all regions of the developing world are widely used. Unlike the heavy use, there are very little information about the health and efficiency of the treatment by these plants. On the other hand, it’s proven that the relationship between apoptosis and cancer has been increased. In the present study the induction of apoptosis as well as its proliferation effects of polygonum avicular extract on Hela-S cervical cancer cell line has been investigated. First, total extract of Polygonum avicular was prepared by n-hexane, methanol and dichloromethane. Then cytotoxic effect of methanolic phase was studied on Hela-S cervical cancer cell line with 12, 24 and 48 h MTT assays. Finally, after determination of IC50, cells were treated with methanolic extract and Hoechest staning assay was done to measure the amount of apoptosis by the extract. Data analysis showed that methanolic extract of Polygonum avicular has dose-dependent cytotoxic effect on Hela-S cervical cancer cell line with IC50= 0.27±0.07 -0.41±0.02 mg/ml and the extract causes apoptosis with time-dependent manner. In conclusion, methanolic extract of polygonum avicular has cytotoxic and apoptotic effect on Hela-S cervical cell line and may be exploited as potential source for developing novel drugs against cervical cancer.

 

Key words: Cervical cancer, polygonum avicular, Hela-S cervical cancer cell line, apoptosis.