African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 408

Full Length Research Paper

Chemical composition and in vitro antioxidant, cytotoxicity activities of Zingiber officinale Roscoe essential oil

Wei Wang1,2, Lin Zhang1,2, Nan Li1,2 and Yuangang Zu1,2,*
1Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Hexing Road 26, 150040, Harbin, P. R. China. 2Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Hexing Road 26, 150040, Harbin, P. R. China.
Email: [email protected]

  •  Accepted: 23 January 2012
  •  Published: 31 March 2012

Abstract

This study was designed to examine the in vitro antioxidant and cytotoxicity activities of Zingiber officinale Roscoe (ginger) rhizome essential oil. GC-MS analysis of the essential oil resulted in the identification of 7 compounds, representing 90.06% of the oil, the major constituents were described as α-zingiberene (52.35%), β-pinene (14.20%) and β-sesquiphellandrene (12.11%). The oil was subjected to screening for their possible antioxidant activity by means of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and β-carotene bleaching test. In the DPPH test system, the concentrations providing 50% inhibition (IC50) values of free radical-scavenging activity of ginger essential oil was determined to be 0.0144% (v/v). In the β-carotene bleaching test system, we tested series concentration of samples to show the antioxidant activities of the oil, the IC50 values of ginger essential oil was 0.55% (v/v). Ginger essential oil exhibited the strong cytotoxicity towards two human cancer cells at low concentration. Its IC50 values on HO-8910 and Bel-7402 were 0.00643 and 0.00256% (v/v), respectively.

 

Key words: Zingiber officinale Roscoe, essential oil, GC-MS, antioxidant activity, cytotoxicity activity.