Evaluation of nutritional, anti-nutritional and some biochemical studies on Pleurotus squarrosulus (Mont.) singer using rats

Duru Majesty; Nwadike Constance; Ezekwe Ahamefula; Nwaogwugwu Caleb; Eboagwu Ijeoma; Odika Prince; Njoku Samuel; Chukwudoruo Chieme

doi:10.5897/AJBR2017.0925

African Journal of
Biochemistry Research

Abbreviation: Afr. J. Biochem. Res.
Language: English
ISSN: 1996-0778
DOI: 10.5897/AJBR
Start Year: 2007
Published Articles: 425

Full Length Research Paper

Evaluation of nutritional, anti-nutritional and some biochemical studies on Pleurotus squarrosulus (Mont.) singer using rats

Duru Majesty

Duru Majesty
Department of Chemical Sciences (Biochemistry Unit), Rhema University, Aba, Nigeria.
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Nwadike Constance

Nwadike Constance
Department of Medical Laboratory Science, Imo State University, Owerri, Nigeria.
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Ezekwe Ahamefula

Ezekwe Ahamefula
Department of Medical Biochemistry, Imo State University, Owerri, Nigeria.
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Nwaogwugwu Caleb

Nwaogwugwu Caleb
Department of Biochemistry, Abia State University, Uturu, Nigeria.
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Eboagwu Ijeoma

Eboagwu Ijeoma
Department of Food Technology, Federal Institute of Industrial Research Oshodi, Nigeria.
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Odika Prince

Odika Prince
Department of Biochemistry, Michael Okpara University of Agriculture, Umudike, Nigeria.
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Njoku Samuel

Njoku Samuel
Department of Biochemistry, Michael Okpara University of Agriculture, Umudike, Nigeria.
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Chukwudoruo Chieme

Chukwudoruo Chieme
Department of Biochemistry, Abia State University, Uturu, Nigeria.
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Article Number - 9E85A2156020
Vol.12(2), pp. 7-27 , February 2018
https://doi.org/10.5897/AJBR2017.0925

Received: 02 January 2017
Accepted: 24 May 2017
Published: 28 February 2018

Copyright © 2024 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0.

References

Adebayo AH, Abolaji AO, Opata TK, Adegbenro IK (2010). Effects of ethanoloic leaf extract of Chrysophyllum albidum G. on biochemical and haematological parameters of albino wistar rats. Afr. J. Biotechnol. 9(14):2145-2150.

Adebayo JO, Adesokan AA, Olatunji LA, Buoro DO, Soladoye AO (2005). Effect of Ethanolic extract of Bougainvillea spectabilis leaves on haematological and serum lipid variables in rats. Biochem.17: 45.

Adedayo MR (2011). Proximate analysis on four edible mushrooms. J. Appl. Sci. Environ. Manage. 15(1):9-11.
Crossref

Adedayo MR, Olasehinde IG, Ajayi A (2010). Nutritional value of edible mushrooms from Egbe farmland, West Yagba Local Government Area, Kogi State. Afr. J. Food Sci. 4(5):297-301.

Adejumo TO, Awosanya OB (2005). Proximate and mineral composition of four edible mushroom species from South Western Nigeria. Afr. J. Biotechnol. 4(10):1084-1088.

Adejumo TO, Coker ME, Akinmoladun VO (2015). Identification and evaluation of nutritional status of some edible and medicinal mushrooms in Akoko Area, Ondo State, Nigeria. Int. J. Curr. Microbiol. Appl. Sci. 4(4):1011-1028.

Adenipekun CO (2008). Bioremediation of engine-oil polluted soil by Pleurotus tuberregium Singer, a Nigerian white-rot fungus. Afr. J. Biochem. 7(1):55-58.

Adeyeye EI, Akinyeye RO, Ogunlade L, Olaofe O, Boluwade JO (2010). Effect of farm and industrial processing on the amino acid profile of cocoa beans. Food Chem. 118:357-363.
Crossref

Agbaire PO, Emoyan OO (2012). Nutritional and Antinutrtional levels of some local vegetables from Delat State, Nigeria. Afr. J. Food Sci. Nutr. 53:189-196.

Agomuo EN (2011). Proximate, Phytochemical and mineral element analysis of the sclerotium of Pleurotus tuberregium. Int. Sci. Res. J. 3:104-107.

Aina DA, Oloke JK, Jonathan SG and Olawuyi OJ (2012). Comparative assessment of mycelia biomass and exo-polysaccharide production in wild type and mutant strains of Scizophyllum commune grown in Submerged liquid medium. Nat. Sci. 10(10):82-89.

Akande AM, Kuforiji OO (2013): Mycelial growth requirement of Pleurotus squarrosulus (Mont.) Singer. Nig. J. Sci. 47:85-91.

Akhtar S, Masood S, Ahmad I, Khan BJ (2012): Effect of cooking on nutritional nutritional composition of mushroom (Pleurotus florida l). Park.J.Food Sci. 22(4):185-187.

Akindahunsi AA, Oyetayo FL (2006). Nutrient and antinutrient distribution of edible mushroom, Pleurotus tuber-regium (fries) singer. ELSEVIER LWT 39:548-553.
Crossref

Akinyele BJ, Obameso JO, Oladunmoye MK. (2011). Phytochemical screening and antimicrobial potentials of three indigenous wild Ganoderma mushrooms from Ondo state, Nigeria. Nig. J. Microbiol. 25:2280-2290.

Aletor VA, Aladetimi OO (1995). Compositional studies on edible tropical species of mushrooms. Food Chem. 54(3):265-268.
Crossref

Alexandar RR, Griffths JM (1933a). Haematocrit in Basic Biochemical methods, 2nd ed., John Wiley and Sons Inc, New York.pp. 186-187.

Alexandar RR, Griffths JM (1933b). Haemoglobin determination by the cyanomethaemoglobin method In: Basic Biochem methods. 2nd ed., John Wiley and Son Inc. New York. Plants of Tropical West African 2nd Edition. pp. 188-189.

Aliyu R, Adebayo AH, Gatsing D, Garba IH (2006). The Effects of ethanolic leaf extract of Commiphora Africana (Bursearaceae) on rat liver and kidney function. J.Pharmacol. Toxicol 2:373-379.

Amadi B, Duru M, Agomuo E, Amadi P, Onedibe O (2017). Nutritional, phytochemicaland sensory evaluation of "Mberiagworagwo" traditional food of Uruagunnewi people in Anambra State, Nigeria. J. Adv. Biol. Biotechnol. 14 (1):1-8.
Crossref

Amadi B, Nchekwube O, Amadi C, Ugbogu A, Duru M. (2013). Elemental, amino acid and phytochemical constituents of three different species of eggplant. Int. J. Med. Arom. Plants. 3(2):200-203.

Amadi BA, Arukwe U, Duru MKC, Adindu EA, Ufornwa EC and Odika PC (2011). The effect of fermentation on anti-nutrients, carbohydrates and vitamin contents of Pentaclethra macrophylla seed. Int. Sci. Res. J. 3:74-77.

Amadi BA, Duru MKC, Agomuo EN (2012). Chemical profiles of leaf, steam, root and flower of Ageratum conyzoides. Asian J. Plant Sci. Res. 2(4):428-432.

Amadi BA, Duru MKC, Ayalogu EOA (2014). Proximate and anti-nutritional composition of "ntiti-ikpa" and "ntubiri" traditional diets of the Ikwerre ethnic national in Nigeria. Eixir Food Sci. 71:24751-24754.

Andrew G, Mirjan S, Juru P (2007). Cultivation techniques and medicinal Pleurotus species. Food Technol. Biotechnol 459(3): 238-249.

Antia BS, Akpan EJ, Okon PA, Umoren IU (2006). Nutritive and anti-nutritive evaluation of sweet potatoes (Ipomoea batatas) leaves. Pak. J. Nutr. 5(2):166-168.
Crossref

AOAC (2006). Official Methods of Analysis of Association of Official Analytical Chemists, 18th ed., AOAC, Arlington, Virgina, USA.

Aremu MO, Basu SK, Gyar SD, Goyal A, Bhowmik PK, DattaBanik S (2009). Proximate Composition and Functional Properties of Mushroom Flours from Ganoderma spp., Omphalotusolearius and Hebelomamesophaeum used in Nasarawa State, Nigeria. Mal. J. Nutr, 15(2):233-241.

Atlas RM, Bartha RC (1992). Hydrocarbon biodegradation and soil spill. Bioremediation. In Marshal K (eds). Adv. Microb. Ecol. NY. 12:287-338.

Bello MO, Farade OS, Adewusi SRA, Olawore NO (2008). Studies of some lesser known Nigerian fruits. Afr. J. Biotechnol. 7:3972-3979.

Beluhan, S, Ranogajec A (2011). Chemical composition and non-volatile components of Croatian wild edible mushrooms. Food Chem. 124:1076-1082.
Crossref

Benjamin AA, Eugene ON, Edward AO (2012). Mineral content and sensory evaluation of "Onunu" and "Mgbam" traditional diet of Ikwerre people of Nigeria. Arch. Appl. Sci. Res. 4(1):330-335.

Boa E (2004). Wild Edible fungi: A global overview of their use and importance to people. Non-wood forest products 17: FAO, Rome.

Bobek P, Ozd'Ä±n L, Galbav'y S. (1998). Dose- and time-dependent hypocholesterolemic effect of oyster mushroom (Pleurotus ostreatus) in rats. Nutrition 14(3):282-286.
Crossref

Breene W (1990). Nutritional and medicinal value of mushrooms. J. Food Prod. 53:883-894.
Crossref

Brunzell JD, Davidson M, Furberg CD, Goldberg RB, Howard BV, Stein JH, Witzturn JL (2008). Lipoprotein management in patient with cardiometabolic risk. J. Am. College Cardiol. 51(15):1512-1524.
Crossref

Buigut SK (2002). Mushroom production in sustainable small-scale farming system-opportunities and constraints: a survey of UasinGishu district. In: Proceedings of the Holticulture seminar on Sustainable Horticultural Production in the Tropics at Jomo Kenyattam University of Agriculture & Technology, Juja, Kenya 3rd - 6th.

Celik I, Suzek H (2008). The hematological effects of methyl parathionin rats. J. Haz. Mat. 153:1117-21.
Crossref

Chan HKM (1981). Consumption of edible mushroom in Hong Kong. Mushroom Newsletter for the Tropics 1(2), 18-22.

Chandravadana MV, Vekateshwarlu G, BujjiBabu CS, Roy TK, Shivashankara K, Meera Pandey, Tewari RP, Selvaraj Y (2005). Volatile flavor components of dry milky mushrooms (Calocybeindica). Flavour Frangance J. 20(6):715-717.
Crossref

Chaney SG (2006). Principles of nutrition I: Macronutrients. In: Textbook of Biochemistry, with Clinical Correlation, 6th ed., Devil. T.M. (ed.). John Wiley and Sons: New York, NY.107-1090. ISBN:100-471-67808-2.

Chang C-T, Miles PG (2008). Mushrooms: Cultivation, nutritional value, medicinal effect, and environmental impact, CRC Press, Boca Raton, Fla, USA, 2nd edition.

Chang ST, Buswell JA (1996). Mushroom Nutriceuticals. World J. Microbiol. Biotechnol. 12:473-476.
Crossref

Cheung LM, Cheung PCK (2005). Mushroom extracts with antioxidant activity against lipid peroxidation. Food Chem. 89:403-409.
Crossref

Chiejina, NV, Olufokunbi JO (2010): Effects of different substrates on the yield and protein content of Pleurotus tuberregium. Afr. J. Biotechnol. 9(11):1573-1577.
Crossref

Dacie JV, Lewis SM (1991). Practical hematology. Churchill Living Stone. Edinburgh. pp. 41-57.

Dahl LK (1972). Salt and hypertension. American J.Clin. Nutr. 25:231-238.
Crossref

David IW (2010). HTMA mineral ratios, a brief discussion of their clinical importance. Trace Elements Newsletter 21:1.

Davies NT, Warrington, S (1986). The phytic acid, minerals, trace elements, protein and moisture content of U.K, Asian immigrant foods. Hum. Nutr. Appl. Nutr. 40A:49-59.

Duru M, Amadi B, Agomuo E, Eze A (2012a). Chemical profile of an anti-malarial concoction "udu" used in Umunchi autonomous community in Isiala Mbano L.G.A of Imo State, Nigeria. J. Emerg. Trends Eng. Appl. Sci. 3(3):444-447.

Duru M, Amadi B, Arukwe U, Adindu E, Amadi C, Onuoha N. (2013a). Fatty acid composition and sensory evaluation of "nduduagworagwo" a local food of Akokwa people in Imo State, Nigeria. Glob. Res. J. Sci. 2(2):67-72.

Duru M, Amadi B, Njoku V (2013b). Evaluation of chemical constituents in "Ji-otor and "Ntubiri-Ikpa" traditional foods of Ikwere ethnic national in Nigeria. Proceedings of the 36th Annual International Conference of Chemical Society of Nigeria 1:38-44.

Duru M, Amadi C, Ugbogu A, Eze A, Amadi B (2012b). Phytochemical, vitamin and proximate composition of Dacryodes edulis fruit at different stages of maturation. Asian J. Plant Sci. Res. 2(4):437-441.

Duru MKC, Agomuo EN, Amadi BA (2012c). Biochemical studies on "udu" an antimalarial concoction used in Umunchi village, Isiala Mbano L.G.A of Imo State, Nigeria. Cont. J. Pharmcol. Toxicol. Res. 5(2):28-34.

Duru MKC, Amadi, BA, Nwadike NC, Ozougwu JC (2015). Anti-nutrients, amino acid quality and performance characteristics of "nduduagworagwo" traditional diets. J. Microbiol. Biotechnol. Food Sci. 4(3):252-256.
Crossref

Edeoga HO, Enata DO (2001). Alkaloids, tannins, and saponins content of some medicinal plants. J. Med. Aromatic Plant Sci. 55:481-504.

Egwim EC, Elem RC, Egwuche RU (2011). Proximate composition, phytochemical screening and antioxidant activity of ten selected wild edible Nigeria mushrooms. Am. J. Food Nutr. 1(12):89-94.
Crossref

Ellis R, Kelsay JL, Reynolds RD, Morris ER, Moser PB, Frazier CW (1987). Phytate:Zinc and Phytate x Calcium: Zinc millimolar ratios in self selected diets of Americans, Asian, Indians and Nepalese. J. Am. Diets Assoc. 87:1043-1047.

Enig MG (1998). Lauric oils as antimicrobial agents: theory of effect scientific rationale and dietary application as adjunct nutritional support for HIV individuals, In Nutrients and food in AIDs, CRC Press, Boca Raton, pp. 81-97.

Ezeibekwe IO, Ogbonnaya CI, Unamba CIN, Osuala OM (2009). Proximate analysis and mineral composition of edible mushrooms in parts of South-Eastern Nigeria. Rep. Opin. 1(4):32-36.

FAO/WHO/UNU (1991). Energy and protein requirements: Report of a joint FAO/WHO/UNU Expert Consultantion. WHO Tech. Rep. Series 724. ISSN: 0512-3054.

Fasidi IO (2008). Substrate sourcing and preparation for mushroom cultivation In: Cultivation of Edible Tropical Mushrooms. (Eds. Fasidi IO, Kadiri M, Jonathan SG, Adenipekun CO, Kuforiji OO) Ibadan University Press. pp. 29-40.

Fekadu H, Beyene F, Desse G (2013). Effect of traditional processing methods on nutritional composition and anti-nutritional factors of anchote (Coccinia Abyssinica (lam.) Cogn) tubers grown in Western Ethiopia. J. Food Process Technol. 4:249.
Crossref

Fereidoon S (2012). Antinutrients and phytochemicals in food. Developed from a symposium sponsored by the Division of Agricultural and Food Chemistry at the 210th National Meeting of the American Chemical Society, Chicago, Illinoi. ACS symposium series, ISSN 0097-6156;2

Fereidoon S (2014). Beneficial health effects and drawbacks of antinutrients and phytochemicals in Foods. Appl. Microbiol. Biotechnol. 97:45–55.

Ferreira ICFR, Vaz JA, Vasconcelos MH, Martins A (2010). Compounds from wild mushrooms with antitumor potential. Anti-Cancer Agents in Med. Chem. 10:424-436.
Crossref

Fife B (2000). The healing miracle of coconut oil. Piccadilly Books ltd. Health wise Publication Colrado Springs, Co. pp. 1-41.

Fillipie M, Umek A (2002): Screening of basidomycetes mushroom extracts for antigenotoxic and biomutagenic activity, Die Pharmazie 57:416-420.

Friedwald TW, Federickson DS, Levy RJ (1972). LDL cholesterol estimation. Clin. Chem.18:499-501.

Fukushima M (2000). LDL receptor mRNA in rats is increased by dietary mushroom (Agaricus bisporus) fibre and sugar beef fibre. J. Nutr. 130:2151-2156.
Crossref

Gemede HF, Ratta N (2014). Antinutritional factors in plant foods: potential health benefits and adverse effects. Int. J. Nutr. Food Sci. 3(4):284-289.
Crossref

Guillamón E, García-Lafuente A, Lozano M (2010). Edible mushrooms: role in the prevention of cardiovascular diseases. Fitoterapia 81(7):715-723.
Crossref

Harbone JB (1973).Phytochemical methods; A guide to modern technique of plant analysis.2nd ed. Chapman and Hall. NY.

Harris RA, Crabb DW (2006). Metabolic interrelationship In: TM Delvin Textbook (Ed.), of Biochem. clin correlations (6th ed.,) New Jersey; John Wiley and Son. pp. 849-046.

Hassan LG, Abdulmumin U, Umar KJ, Ikeh PO, Aliero AA (2014). Nutritional and anti-nutritional composition of Strychnos innocua Del. (Monkey Orange) fruit pulp grown in Zuru, Nigeria. Nig. J. Basic Appl. Sci. 22(1&2):33-37. Crossref

Hassan LG, Bagudo BU, Aliero AA, Umar KJ, Abubakar L, Sani NA (2011). Evaluation of nutrient and anti-nutrient contents of Parkia biglobosa (L.) Flower. Nig. J. Basic Appl. Sci. 19(1):76- 80. Crossref

Hayes WA (1997). Mushrooms: Single cell protein from cellulose. In: Annual Report on Fermentation Processes, (D. Pedman) Academic Press, New York. P 8.

Heleno SA, Barros L, Martins A, Queiroz MJ, Santos-Buelga C, Ferreira IC (2012). Phenolic, polysaccharidic, and lipidic fractions of mushrooms from northeastern Portugal: Chemical compounds with antioxidant properties. J. Agric. Food Chem. 60:4634-4640. Crossref

Heleno SA, Barros L, Sousa MJ, Martins A, Ferreira ICFR (2010). Tocopherols composition of Portuguese wild mushrooms with antioxidant capacity. Food Chem. 119(4):1443-1450. Crossref

HMSO UK (1994). Department of Health. Nutritional aspects of cardiovascular disease. London. Rep. Health Soc. Sub. 46:37-46.

Hussein JM, Tibuhwa DD, Mshandete AM, Kivaisi AK (2014). Molecular phylogeny of saprophytic wild edible mushroom species from Tanzania based on ITS and nLSUrDNA sequences. Curr. Res. Environ. Appl. Mycol. 4:250-260.

Ibanga OI, Okon DE (2009). Minerals and antinutrients in two varieties of African pear (Dacryodes edulis). J. Food Technol. 7:106-110.

Igwe CU, Ojiako OA, Anugweje KC, Nwaogu LA, Ujowundu CO (2012). Amino acid profile of raw and locally processed seeds of Prosopls africana and Ricinus communis: Potential antidotes to protein malnutrition. Funct. Foods Health Dis. 2(4):107-119.

Ijeh FI, Okwujiako IA, Nwosu PC, Nnodim H. (2009). Phytochemical composition of Pleurotus tuber regium and effect of its dietary incorporation on body/organ weights and serum triacylglycerols in albino mice. J. Med. Plants Res. 3(11):939-943.

Ikewuchi CC, Ikewuchi JC (2009). Chemical profile of Pleurotus tuber-regium(Fr.) Sing's sclerotia. Pac. J. Sci. Technol. 10(1):295-299.

Isikhuehmen O, Anoliefo G, Oghale O (2003). Bioremediation of crude oil polluted soil by the white rot fungus Pleurotus tuberregium(Fr.) Sing. Environ. Sci. Pollut. Res. 10:108-112. Crossref

Isikhuemhen SO, Le Bauer DS (2004). Growing Pleurotus tuberregium. Mushworld Publication, 11:264-274.

Jain NC (1986).Veterinary Hematology (Jain NC Ed) Lea and Ferbiger, Philadelphia.

Jiskani MM (2001). Energy potential of mushrooms. The DAWN Economic and Business Review, Oct. 15-21, 2001. p. 4.

Jonathan SG (2002). Studies on vegetative growth requirements and antimicrobial activities of higher fungi from Nigeria. Ph.D thesis University of Ibadan, Nigeria.

Jonathan SG, Popoola KOK, Olawuyi OJ, Ajiboye M, Oyelakan AO (2012). Insect and fungal pests of some mushrooms collected from university of Ibadan, Nigeria campus. Nature and Science, 10(9):142-147.

Kadiri M, Fasidi IC (1990). Studies on enzyme activities of Plerotus tuber regium Hein at various fruit body stages. Nahrung, 34(8): 695-999. Crossref

Kadiri M, Fasidi IO (1992). Secondary plant products in some Nigerian mushrooms. Nig. J. Bot. 5:187-192.

Kalac P (2009). Composition and nutritional value of European species of wild growing and edible mushrooms. Pak. J. Bot. 38:799-804.

Kayode RMO, Olakulehin TF, Annongu AA, Sola-Ojo, FE, Oyeyinka SA, Kayode BI (2013). Evaluation of the nutritional composition and phytochemical screening of an exotic and wild species of oyster mushrooms (Pleurotus-sajorcaju). Nig. J. Agric. Food Environ. 9(3):48-53.

Khurdishul Z, Sargamay B, Immamul SM (2010). Proximate composition and mineral content of selected edible mushroom varieties of Bangladesh. Bangladesh J. Nutr. pp. 22-23.

Kirk PM, Canon DW, Staiper JA (2008). Dictionary of the fungi 10th Edition. CABI ISBN 10: 0851998267.

Krishan AG, Veena G (1980). 2, 4-4. triminazo benzene – induced haematobiochemical anomalies in fish (Chanma-puntatus Bull). Environs Contam.Toxicol. 25:136-141. Crossref

Kuforiji OO (2008). Use of Mushrooms in novelty medicine. In: Fasidi IO, Kadiri M, Jonathan SG, Adenipekun CO, Kuforiji OO (eds.) Cultivation of edible tropical mushrooms Ibadan University Press, Nigeria.

Kuforiji OO, Fasidi IO (1998). Growth of P. tuber-regiuma Nigerian Mushroom. Proceedings of Nigerian Society for Microbiology. (1): 73-78. Fed. Institute of Industrial Res. Oshodi. Dec. 1989.

Kuforiji OO, Fasidi IO, Olatunji, OO (2003). Production of the oyster mushroom P.tuber-regium) from agro industrial wastes. Nig. J. Microbiol. 17 (1): 68-70.

Lillian HM (2004). Food chemistry. 4th edition. CBS Publishers and Distributors Pvt. Ltd.: New Delhi, India.12-27, 66-79, 114.

Lisa DF, Paula RG (2010): Omega-3 amd Omega-6 fatty acids. Nebguide, University of Nebraska, Lincoln Extension, Institute of Agric. Nat.Res. View (Accessed on 23/05/2013).

Lisa N (2008). Making sense of cholesterol ratios, lipid profiles and your heart disease risk scores. View (Accessed on 6/05/2016).

Maria EV, Talia H, Octavio P (2015): Edible mushrooms; improving human health and promoting quality life. Int. J. Microbiol. Volume 2015.Article ID 376387.

Martirosyan DM, Miroschnichenko LA, Kukoawa SN, Pogojeva AV, Zoloedov VI (2007). Amaranth oil application for heart disease and hypertension. Lipid Health Dis. 6:1. Crossref

Mary KC, Shawn OF (2006). Biochemistry (5th ed.). Cengage Learning. P 579. ISBN 0-534-40521-5.

Millán J, Pinto X, Munoz A, Zuniga M, Rubies-Prat J, Pallardo FL, Masana L, Mangas A, Hernandez-Mijares A, Gonzales-Santos P, Ascaso FJ, Pedro-Botet J (2009).Lipoprotein ratios: Physiological significance and clinical usefulness in cardiovascular prevention. Vas. Health Risk Manage. 5:758-765.

Mitchikpe ECS, Dossa RAM, Ategbo EAO, Vanraaiji JMA, Hulshof PJM Kok FJ (2008). The Supply of bioavailable iron and zinc may be affected by phytate in Beninese Children, J. Food Comp. Anal. 21:17-25. Crossref

Mlitan AM, Sasi MS Alkherraz AM (2014). Proximate and minor mineral content in some selected basil leaves of Ocimum gratissimum L, in Libya. Int. J. Chem. Eng. Appl. 5:8-17.

Motey GA, Odamtten GT, Akaho EHK, Opata NS (2015). Elemental and mycochemical profile, antimicrobial activities and uses of the sclerotium of Pleurotus tuberregium, a medicinal mushroom in Ghana. Canadian J. Pure Appl. Sci. 9(3):3535-3540.

National Institute of Health (1985). Guide for the care and use of laboratory animals". U.S. Department of Health Education and Welfare, NIH Publication. Washington, D.C. pp. 85-123.

National Research Council (NRC) (1989). Recommended Dietary Allowances. 10th edn., National Academy Press, Wasington D.C., USA.

Nkafamiya II, Oseameahon SA, Modibbo UU, Haggi D (2010). Vitammins and effect of blanching on nutritional and anti-nutritional values of non-conventional leafy vegetable. Afr. J. Food Sci. 4(6):335-341.

Oei P, Nieuwenhuijzen BV (2005). Small scale mushroom cultivation. Agrodok 40. Agromisa Foundation and CTA, the Netherlands, P 86.

Ojiako OA, Akubugwo EI (1997). An Introductory approach to practical Biochemistry. CRC Publishers, Owerri, Imo Sate, Nigeria P 67.

Okaka JC, Okaka ANC (2001). Food composition, spoilage and shelf life extension, OCJARC. Acadamic Publishers, Enugu, Nigeria.

Okaka JC, Okaka ANC (2005). Foods: composition, spoilage, shelf-life extension, 3rd OcJANCO Academic Publishers. pp. 50-120.

Okhuoya JA, Etugo JE (1993). Studies of the cultivation of Pleurotus tuber-regium(FR) Sing. an edible mushroom. Bioresour. Technol. 44:1-3. Crossref

Okhuoya JA, Okogbo FO (1990). Induction of edible sclerotia of Pleurotus tuber-regium(FR) Sing. in the laboratory. Ann. Appl. Biol. 117:295-298. Crossref

Okhuoya JA, Okogbo FO (1991). Cultivation of Pleurotus tuber-regium(Fr) Sing on Various Farm Wastes. Proc. Oklahoma Acad. Sci. 71:1-3.

Okwu DE (2005). Phytochemical,vitamin and mineral contents of two Nigeria medicinal plants. Int. J. Mol. Med. Adv. Sci. 1(4): 375-381.

Okwulehie IC, Odunze EI (2004). Evaluation of the nutritional value of the myco-chemical and mineral compositionof some tropical edible mushrooms. J. Sustainable Agric. Environ. 6(2):163-170.

Okwulehie IC, Ogoke JA (2013). Bioactive, nutritional and heavy metal constituents of some edible mushrooms found in Abia state of Nigeria. Int. J. Appl. Microbiol. Biotechnol. Res. 1:7-15.

Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lilly P, Sander J, Spies G, Bracken W, Dorato M, Deun KV, Smith P, Berger B, Heller A (2000). Concordance of toxicity of pharmaceuticals in humans and in animals. Regul. Toxicol. Pharmacol. 32:56-67. Crossref

Olusanya JO (2008). Essential of food and nutrition.1st edition Apex book limited, pp.36-76.

Omar NAM, Abudullah N, Kuppusamy UR, Abdulla MA, and Sabaratnam V (2011). Nutritional composition, antioxidant activities, and antiulcer potential of Lentinus squarrosulus (Mont.) Mycelia extract. Evidence-Based Complementary and Alternative Medicine. Volume 2011. Article ID 539356, 8p.

Onuoha CI (2007). Cultivation of the mushroom (Pleurotus tuberregium) using some local substrates. Life Sci. J. 4:58-61.

Onwuka (2005). Food analysis and instrumentation: theory and practice. Napthali prints, Lagos, Nigeria. pp. 64-118.

Onyeike EN, Ehirim FC (2001). Chemical and sensory evaluation of melon fungus (Pleurotus tuber regium) and melon fungus cake. Nig. J. Biochem. Mol. Biol. 16(1):77-81.

Paul G, Pearson S (2005). The vitamins, 2nd edn., Academic Press. New York. USA. pp. 31-34.

Rang HP, Dale MM, Ritter JM, Moore PK (2005). Parmacology, 5th edition.Elsevier. India. ISBN.81-8147-917-3.

Rodriguez-Cruz M, Tovar AR, Del Prado M, Torres N (2005). Molecular mechanisms of action and health benefits of polyunsaturated fatty acids. Rev. Invest. Clin. 57(3):457-72.

Scientific Advisory Committee on Nutrition (SACN) (2008). Draft SACN position statement on dietary fibre and health and the dietary fiber definition. August 2008. SACN/08/20. View

Sampath H, Ntambi JM (2005). Polyunsaturated fatty acid regulation of genes of lipid metabolism. Annu. Rev. Nutr. 25:317-340. Crossref

Schneider G, Wolfling J (2004). Synthesis cardenolides and related compounds. Curr. Org. Comp. 8:14.

Soetan KO, Olaiya CO, Oyewole OE (2010). The importance of mineral elements for humans, domestic animals and plants: a review. Afr. J. Food Sci. 4:200-222.

Speckman DH, Stem EH, Moore S (1958). Automatic recording apparatus for use in the chromatography of amino acids. Anal. Chem. pp. 1190-1191. Crossref

Stevenson JA, Lentz PL (2007). Mushroom. Microsoft® Student 2008 with Encarta Premium 2008 (DVD). Redmond, WA: Microsoft Corporation.

Ugbogu AE, Amadi BA (2014). Biochemical composition of month beans (V.aconitifolia) and pidgeon pea (C.cajan). Intraspec. J.Nutri.Food Sci. 1 (1): 001-008.

Ullah RJA, Khader I, Hussain NM, Talha AM, Khan N (2012). Investigation of macro and micronutrients in selected medicinal plants. Afr. J. Pharm. Pharmacol. 69:1829–1832.

Urga K, Narasimba HV (1998). Phytae:zinc and phytate×calcium:zinc molar ratios in selected diests of Ethiopans. Bull. Chem. Soc. Ethiop. 12(1):1-7. Crossref

Usoro CAO, Adikwuru CC, Usoro IN, Nsowu AC (2006). Lipid profile of posimenopausal women in Calabar, Nigeria. Pak. J. Nutr. 5(1):79-82. Crossref

Uwakwe AA, Ayalogu EO (1998). Proteins, In: Biochemistry (A tropical approach), FIUS Publishers, Port Harcourt. 2:44-45.

Valvi SR, Rathod VS (2011). Mineral composition of some wild edible fruits from Kolhapur district. Int. J. Appl. Biol. Pharmaceut. Technol. 2:112-119.

Wardlaw CM, Kessel MW (2002). Perspective in nutrition (5th ed.). Boston: McGraw-Hill.

Woldegiorgis AZ, Abate D, Haki GD, Ziegler GR (2015). Major, minor and toxic minerals and anti-nutrients composition in edible mushrooms collected from Ethiopia. Food Process Technol. 6:134-142.

Yakubu MT, Akanji MA, Oladiji AT (2007). Haematological evaluation in male albino rats following chronic administration of aqueous extract of Fadogia agrestis stem. Pharmacog. Mag.3:34.

Young VR, Pellet PL (1990). Current concept concerning indispensable amino acids in adults and their implications for international nutrition planning food. Nutr. BulI. 12:289-300.