African Journal of
Biochemistry Research

  • Abbreviation: Afr. J. Biochem. Res.
  • Language: English
  • ISSN: 1996-0778
  • DOI: 10.5897/AJBR
  • Start Year: 2007
  • Published Articles: 381

Table of Content: June 2019; 13(5)

June 2019

Partial purification of invertase from Momordica charantia (bitter melon) by three phase partitioning (TPP) method

The single step three-phase partitioning (TPP) method was evaluated for the purification of invertase from Momordica charantia. Optimum conditions for TPP method, that is, 70% ammonium sulfate saturation, 1:0.5 t-butanol ratio and 30 min incubation time lead to 20.28% efficiency and 10.48 fold partial purification. Total protein decreased from 34.86 mg to 0.68 mg in the homogenate, and the specific activity of the...

Author(s): Nihan Kübra Belligün and Burcu Saygıdeğer Demir  

June 2019

Cadmium-induced toxicity and antioxidant enzyme responses in tissues and organs of African catfish (Clarias gariepinus)

Juveniles of Clarias gariepinus were exposed to different concentrations of cadmium chloride for 96 h under laboratory conditions using static bioassays with continuous aeration to determine its mean lethal concentration (LC50), biochemical alterations, bioaccumulation and histological pattern in a sub-lethal toxicity test. The median lethal concentration (LC50) at the end of the acute toxicity was 120.2 mgL-1. Also the...

Author(s): Chisom P. OSISIOGU and Omolara T. ALADESANMI