African Journal of
Environmental Science and Technology

  • Abbreviation: Afr. J. Environ. Sci. Technol.
  • Language: English
  • ISSN: 1996-0786
  • DOI: 10.5897/AJEST
  • Start Year: 2007
  • Published Articles: 1076

Full Length Research Paper

Ecotoxicological effects of discharge of Nigerian petroleum refinery oily sludge on biological sentinels

Atuanyan Ernest
  • Atuanyan Ernest
  • Department of Microbiology, Faculty of Life Sciences, University Of Benin, Benin City, Edo State, Nigeria
  • Google Scholar
Tudararo-Aherobo Laurelta
  • Tudararo-Aherobo Laurelta
  • Department of Microbiology, Faculty of Life Sciences, University Of Benin, Benin City, Edo State, Nigeria
  • Google Scholar

  •  Received: 07 April 2014
  •  Accepted: 22 December 2014
  •  Published: 08 February 2015


American Public Health Association (APHA) (1998). Standard, Methods for the Examination of Water and Waste Water 20th Edition Published by American Public Health Association, Washington DC. 1220p.
American Society for Testing and Materials (ASTM). Standard E 2172-01 (2001). Standard guide for conducting laboratory soil toxicity with nematode Caenorhabditiselegans. Annual Book of ASTM Standard 11: 1606-1616.
Atlas RM, Bartha R (1992). Hydrocarbon biodegradation and oil spill bioremediation; in KC Marshall (Ed). Advances in Microbial Ecology. New York. Plenum Pres. Vol. 12, pp. 287-338.
Buikema AL, Nederlehner BR, Cairn J (1982). Biological Monitoring Part IV – Toxicity Testing. Water Resources 16:239-262.
Colwell RR, Zambruski MS (1972). Methods of Aquatic Microbiology University Park Press, Baltimore. 461p.
Davies NA, Hodson ME, Black S (2003). The influence of time on lead toxicity and bioaccumulation determined by the OECD earthworm toxicity test. Environ. Pollut. 121(1):55-61.
Department of Petroleum Resources (DPR) (2002). Environmental Guidelines and Standards for the Petroleum Industry in Nigeria (EGASPIN) 314p.
Dokaniakis SN, Kornaros M, Lyberatos C (2005). On the effect of xenobiotic bacterial nitrite oxidation. Proceedings of the 9th International Conference on Environmental Sciences and Technology Rhodes Island, Greece.
Doube BM, Schmidt O (1997). Can the abundance or activity of soil macrofauna be used to indicate the biological health of soils? In: Biological Indicators of Soil Health. Eds. C. Pankhurst, B Doube and V. Gupta. CAB International New York. pp. 265-295.
Duffus JH (1980). Environmental Toxicology (Resource and Environmental Science Series). Arnold Publishers Ltd. 26p.
Edwards WM, Shipitalo MJ (1998). Consequences of earthworms in agricultural soils; Aggregation and porosity. In Earthworm Ecology (Eds C.A. Edwards) St. Lucie Press Boca Raton, Florida. pp. 147- 161.
Englert CJ, Kenzie EJ, Dragun J (1993) . Bioremediation of petroleum products in soil, Principles & Practice of Petroleum Contamination, in soils. API, 40:111-129.
Environment Canada (1992). Biological Test Method; Acute test for sediment toxicity using fresh and brackish or estuarine amplipods. Conservation and protection, Ottawa, Ontario Report EPSI /RM/26.
Finney DJ (1978). Probit Analysis, Cambridge University Press, London. 333p.
Grunditz C, Dalhammar G (2001). Development of nitrification inhibition assays using pure cultures of Nitrosomonas and Nitrobacter. Water Res. 35:433-440.
Hazardous Waste Management System (1997). Environmental Protection Agency 62:29-32.
Krab J, Mathes K, Breckling B, Lonker O, Schulz – Berend V (2000). Scale – up biologischer Reinigungsvertahren – projeckt – Abachlubericht, bmb. F; Bremen.
Ma X, Ortolano L (2002). Environmental regulation in China, Rowman and little field. Publishers, Inc . 209p.
MacGeer JC, Brix KV, Skeaff JM, Deforest DK, Brigham SI (2003). Inverse relationship between bioconcentration factor and exposure concentration for metals, implication for hazard assessment of metals in the aquatic environment. Environ. Toxicol. Chem. 22:1017-1037.
Mishra S, Jyot J, Kuhad RC, Lal B (2001). Evaluation of inoculums addition to stimulate in – situ bioremediation of oily – sludge contaminated soil. Appl. Environ. Microbiol. 67:1675-1681.
Okpokwasili GC, Odokuma LO (1997). Response of Nitrobacter to oilfield dispersants and domestic detergents. Trop. Freshw. Biol. 6:65-74.
Oolman T, Baker RR, Rentro NL, Marshall GE (1996). Refinery Uses Bioslurry Process to Treat RCRA Wasters. Hydrocarb. Process. 71(76):18-20.
Organization for economic co-operation and Development (OECD) (2002). Guidelines for testing of chemical TG 216. C. 21 Soil microorganisms; Test Nitrogen Transform.
Organization for economic cooperation and Development (OECI) (2004) Guidelines for the Testing of Chemicals No 218; Sediment – water chironomid toxicity test using spiked sediment (Adopted April 2004).
Prospt TL, Lochmiller RI, Quals CW, Mcbee K (1999). In-situ (mesocosm). Assessment of immunotoxicity risks to small mammals inhabiting petrochemical waste sites. Chemosphere 38:1049-1062.
Spiegel H (2002). Trace element accumulation is selected bioindicators exposed to emission along the industrial facilities of Danube, lowland. Turk. J. Chem. 26:815-823.
Suschka J, Mrowies B, Kuszmider G (1996). Respose of Nitrobacter toxicity of oil field dispersants and domestic detergents. Trop. Freshw. Biol. 6:65-74.
Tang J, Lu X, Sun Q, Zhu W (2012). Aging effect of Petroleum Hydrocarbon in Soil under different Attenuation Conditions. Agric. Ecosyst. Environ. 149:109-117.
Terhivuo J, Pankakoski E, Hyvarinen H, Koivisto K (1994). PB uptake by ecologically dissimilar earthworms (Lumbricidae) species near a lead smelter in Southern Finland. Environ. Pollut. 85:87-96.
United State Environmental Protection Agency (USEPA) (2004). United States Environmental Protection Agency, understanding oil spills and oil spill response. htt://
Ururahy AFP, Marins MDM, Vital RL, Therezinha I, Pereira N (1998). Effect of Aeration on Biodegradation of Petroleum Wastes. Rev. Microbiol. 29:4.
Wang W (1984) Time response of Nitrobacter to toxicity. Environ. Int. 10:21-24.
Wang W, Reed P (1984). "Nitrobacter bioassay for aquatic toxicity." Toxicity screening procedures using bacterial systems. 309-325.
Wilde EW, Soracce RJ, Mayack LA, Shealy RL, Broadwell TL, Steffen RF (1983). Comparison of chlorine and chlorine dioxide toxicity to fathead Minnows and Bluegill. Water Res. 17(10):1327-1331.
Williamson KJ, Johnson OG (1981). A bacterial bioassay for assessment of wastewater toxicity. Water Res. 15:383-390.