Full Length Research Paper
Abstract
Transacylation reaction of citrus pectin catalyzed by pectin methyl esterase (PME) from Aspergillus niger observed by laser particle size analyzer was investigated. PME was purified by a highly methoxylation of cross-linked alcohol-insoluble solids (HM-CL-AIS) column chromatography and a subsequent Sephadex G-75 column chromatography from commercial pectic enzyme (CPE) of A. niger. The increases ofparticle size (transacylation reaction) in PME-treated pectin solution are pectin content and PME activity dependent and expressed a maximum at 0.3% pectin and 0.5 U/ml PME, respectively. PME activity (de-esterification reaction) was highly stable below 50°C but lost completely when above 70°C, while the particle size of PME-treated pectin solution decrease at temperature above 40°C. PME activity was optimum at pH 5.0, while the increase of particle size in PME-treated pectin solution reaches a maximum at pH 3.5. The different characteristics between PME from A. niger and the published plant PME reveal that there are at least two kinds of PME-catalyzed transacylation reaction, one for plant PME and the other for microbial PME.
Key words: Transacylation, pectin methyl esterase, Aspergillus niger.
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