African Journal of
Food Science

  • Abbreviation: Afr. J. Food Sci.
  • Language: English
  • ISSN: 1996-0794
  • DOI: 10.5897/AJFS
  • Start Year: 2007
  • Published Articles: 924

Full Length Research Paper

Isolation and characterization of fungal strains from the seeds of Bambara groundnut (Vigna subterranea (L.) Verdcourt) produced in Burkina Faso

OUILI Amidou S.
  • OUILI Amidou S.
  • Laboratoire de Microbiologie et de Biotechnologie Microbienne, Université Joseph KI-ZERBO 03 BP 7021 Ouagadougou 03, Burkina Faso
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MAIGA Ynoussa
  • MAIGA Ynoussa
  • Laboratoire de Microbiologie et de Biotechnologie Microbienne, Université Joseph KI-ZERBO 03 BP 7021 Ouagadougou 03, Burkina Faso
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ZIDA Elisabeth Pawindé
  • ZIDA Elisabeth Pawindé
  • Laboratoire de Phytopathologie et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), Centre National de la Recherche Scientifique et Technologique (CNRST), 01 BP 476, Ouagadougou, Burkina Faso.
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OUOBA Adjima
  • OUOBA Adjima
  • Centre Universitaire Polytechnique de Ziniaré, Université Joseph KI-ZERBO 03 BP 7021 Ouagadougou 03, Burkina Faso.
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NANDKANGRE Hervé
  • NANDKANGRE Hervé
  • Centre Universitaire de Tenkodogo, Université Thomas SANKARA, 12 BP, Ouagadougou 12, Burkina Faso.
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COMPAORE Cheik Omar Tidiane
  • COMPAORE Cheik Omar Tidiane
  • Laboratoire de Microbiologie et de Biotechnologie Microbienne, Université Joseph KI-ZERBO 03 BP 7021 Ouagadougou 03, Burkina Faso
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NIKIEMA Mahamadi
  • NIKIEMA Mahamadi
  • Laboratoire de Microbiologie et de Biotechnologie Microbienne, Université Joseph KI-ZERBO 03 BP 7021 Ouagadougou 03, Burkina Faso
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OUEDRAOGO Mahama
  • OUEDRAOGO Mahama
  • Laboratoire de Génétique et de Biotechnologie Végétales, Institut de l'Environnement et de Recherches Agricoles (INERA), 04 BP 8645 Ouagadougou, Burkina Faso.
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OUATTARA Aboubakar Sidiki
  • OUATTARA Aboubakar Sidiki
  • Laboratoire de Microbiologie et de Biotechnologie Microbienne, Université Joseph KI-ZERBO 03 BP 7021 Ouagadougou 03, Burkina Faso
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  •  Received: 04 January 2022
  •  Accepted: 21 April 2022
  •  Published: 31 May 2022

Abstract

Bambara groundnut is the main food legume after cowpea, especially in rural areas in Burkina Faso. Due to inappropriate production and storage conditions, the seeds are susceptible to contamination by several fungi. Therefore, the present study was conducted to isolate and identify the postharvest fungi associated with Bambara groundnut seeds produced in Burkina Faso. To do this, a total of 99 seed samples were collected in the three agro ecological zones of Burkina Faso. The sanitary analysis of the seeds was carried out using the blotting paper method with slight modifications. The isolation and purification of the isolates was performed on Potato Dextrose Agar medium while their identification was done through macroscopic and microscopic phenotypical characterization using different culture media (Malt Extract Agar and Czapeck Dox Agar) and different identification keys. A total of 421 fungal strains were isolated and the predominant genera were Aspergillus belonging to section flavi (66.84%), nigri (59.04%), and Macrophomina (26.49%). This study shows that Bambara groundnut seeds produced in Burkina Faso are contaminated by several fungal strains and that seed infection rates by these fungi differ according to the agro-ecological zones. Post-harvest and storage techniques need to be improved to limit crop losses.

 

Key words: Agro-ecological zone, Bambara groundnut, seeds, fungal strains, macroscopic and microscopic characteristics, Burkina Faso.