African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 4962

Full Length Research Paper

Host and tissue preferences of Enterobacter cloacae and Bacillus amyloliquefaciens for endophytic colonization

Zayda Piedad Morales Moreira
  • Zayda Piedad Morales Moreira
  • 1. Center of Agricultural, Environmental and Biological Sciences (CCAAB), Federal University of Reconcavo da Bahia (UFRB), 44380-000 Cruz das Almas, BA, Brazil; 2. Faculty of Engineering and Agricultural Sciences, Universidad de las Americas, 170122 Quito, Ecuador.
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Elizabeth Amelia Alves Duarte
  • Elizabeth Amelia Alves Duarte
  • Center of Agricultural, Environmental and Biological Sciences (CCAAB), Federal University of Reconcavo da Bahia (UFRB), 44380-000 Cruz das Almas, BA, Brazil
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Thiago Alves Santos de Oliveira
  • Thiago Alves Santos de Oliveira
  • Center of Agricultural, Environmental and Biological Sciences (CCAAB), Federal University of Reconcavo da Bahia (UFRB), 44380-000 Cruz das Almas, BA, Brazil
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Fernando Pereira Monteiro
  • Fernando Pereira Monteiro
  • Phytopathology Department, Federal University of Lavras (UFLA), 37200-000 Lavras, MG, Brazil
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Leandro Lopes Loguercio
  • Leandro Lopes Loguercio
  • Department of Biological Sciences (DCB), State University of Santa Cruz (UESC) , 45662-900 Ilheus, BA, Brazil
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Jorge Teodoro de Souza*
  • Jorge Teodoro de Souza*
  • Phytopathology Department, Federal University of Lavras (UFLA), 37200-000 Lavras, MG, Brazil
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  •  Received: 11 March 2015
  •  Accepted: 18 May 2015
  •  Published: 20 May 2015

Abstract

Enterobacter and Bacillus are common endophytes in many plants. Endophytic Enterobacter cloacae 344 and Bacillus amyloliquefaciens 629 from cacao had their colonization patterns assessed in different plants and organs. The 344’s colonization in all plants was ~12x higher than those observed for 629. CFU countings were 16 and 3 times lower for 344 and 629, respectively, in non-sterile than in sterile conditions. The results suggested that 344 is a better endophytic colonizer than 629, but this seems a better competitor than 344. Indigenous endophytic fungi interfered with the colonization levels of both strains in all plants. Plant species, tissue and competing microbes appear to define the levels of endophytic colonization by these bacteria.

Key words: Cacao, endophytic bacteria, population densities.