African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5223

Short Communication

Flagellin complete sequence as an inter-specific molecular phylogenetic marker among bacteria

Ernesto Borrayo1,2, M. D. Martinez-Pena1, J.A. Morales-Valencia2, M. Cortes-Cruz1and R. Arteaga-Garibay1*
1Centro Nacional de Recursos Genéticos, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. 2Departamento de Ciencias Computacionales, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara
Email: [email protected]

  •  Accepted: 15 February 2013
  •  Published: 19 February 2013

Abstract

Flagellin, a flagelar subunit protein, presents four structural domains, two of which are highly conserved among bacteria, while the other two tend to be variable. Flagellins have been used as phylogenetic molecular markers, mainly intra-specifically. In this work, a marker comparison for bacterial phylogenetic reconstruction was performed by means of five different DNA sequences: the conserved and complete sequence of flagellins, standard 16S rRNA and recA molecular markers, as well as coding S16 sequence. Results showed that flagellin complete sequence may be an accurate phylogenetic marker for phylogenetic reconstruction among bacteria.

 

Key words: Flagellin, molecular phylogenetic marker, tree comparison.