African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Detection and identification of groundwater bacteria in Sebha City, Libya

Salem, A. Mahfouz1 F. M. Shaieb2 and A. E. K Elzen1
1Botany Department, Faculty of Science, Sebha University, Libya. 2Botany Department, Faculty of Science, Omar Almukhtar University, Libya.
Email: [email protected]

  •  Accepted: 08 August 2008
  •  Published: 31 August 2008

Abstract

Drinking water systems supplied by untreated groundwater were examined to determine whether coliform or heterotrophic plate count bacteria are capable of growing. Filterable bacteria were present in 42% of the 46 groundwater sources examined by using nonselective media (R2A and full strength m-HPC agars). Pseudomonads were the most frequently identified group of filterable bacteria detected. FlavobacteriumAlcaligenesAcinetobacter and Achromobacter isolates were also identified. Total coliforms were recovered only from six samples taken from wells near the wastewater lagoon, following filtration through 0.45-µm-pore-size membrane filters by using selective M-Endo LES agar or mT7 agar. In addition, none of the isolates identified from nonselective media were coliforms. Similarly, neither total coliforms nor specifically Escherichia coli were detected in these filtrates when Colilert P/A medium was used.

 

Key words: ground water, contamination, Flavobacterium, Alcaligenes, Acinetobacter, AchromobacterEscherichia coli.