African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Direct detection of Brenneria rubrifaciens by PCR-based assay using rubrifacine synthetic gene

Shree Prasad Thapa1, Chun Keun Lim2, Sam Kyu Kim2, Jun Mo Cho1, Jang Hyun Hur1 and Duck Hwan Park2*
  1Department of Biological Environment, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Korea. 2Department of Applied Biology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Korea.
Email: [email protected]

  •  Accepted: 02 August 2010
  •  Published: 18 August 2010

Abstract

 

A rapid high-throughput, specific and sensitive PCR-based assay coupled with DNA hybridization technique for the detection of deep bark canker (DBC) pathogenBrenneria rubrifaciens directly from mixed bacterial cells and in planta was developed. These result shows that the specific genetic markers have a powerful potential to detect B. rubrifaciens directly from crude samples at field conditions.

 

Key words: Brenneria rubrifaciens, deep bark canker, PCR, rubrifacine.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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