Abstract

In this paper, adopting orthogonal design method, the Morchella esculenta mycelia from Qinghai-Tibetan plateau was preliminary studied in different growing conditions such as pH, temperature, carbon sources, nitrogen sources and growth factors. The results showed that the M. esculenta mycelium liquid fermentation pH was between 6.0 to 7.0, cultivating optimum temperature 24ËšC, training required for 1.2% of potassium nitrate nitrogen (KNO₃), carbon source was 4.5% soluble starch, growth factor biotin was 0.1%. At the same time, we also took the plateau M. esculenta polysaccharide fermentation liquid extraction, the extraction time was optimal combination 2 h, precipitated with ethanol concentration of 90% extraction ratio 1:20, the highest yield of polysaccharides.

Key words: Qinghai-Tibetan Plateau, Morchella esculenta, sporocarp, mycelium, static culture.