African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Antimicrobial and antitumor activities of crude secondary metabolites from a marine fungus Penicillium Oxalicum 0312F1

Shuo Shen
  • Shuo Shen
  • 1. Qinghai Academy of Agriculture and Forestry, Xi-ning, Qinghai, China. 2. The Tibet Plateau Biotechnology Key Laboratory of Ministry of Education, Xi-ning, Qinghai, China. 3. State Key Laboratory, Breeding Base-Key Laboratory of Qinghai Province for Plateau Crop Germplasm Innovation and Utilization, China.
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Wei Li
  • Wei Li
  • 1. Qinghai Academy of Agriculture and Forestry, Xi-ning, Qinghai, China. 2. The Tibet Plateau Biotechnology Key Laboratory of Ministry of Education, Xi-ning, Qinghai, China. 3. State Key Laboratory, Breeding Base-Key Laboratory of Qinghai Province for Plateau Crop Germplasm Innovation and Utilization, China.
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Jian Wang*
  • Jian Wang*
  • 1. Qinghai Academy of Agriculture and Forestry, Xi-ning, Qinghai, China. 2. The Tibet Plateau Biotechnology Key Laboratory of Ministry of Education, Xi-ning, Qinghai, China. 3. State Key Laboratory, Breeding Base-Key Laboratory of Qinghai Province for Plateau Crop Germplasm Innovation and Utilization, China.
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  •  Received: 11 December 2013
  •  Accepted: 10 March 2014
  •  Published: 02 April 2014

Abstract

The extract of fermentation product of the P. oxalicum strain 0312F1 was isolated through gel (Sephadex LH-20) column chromatography to give fractions A, B, C, D, E and F. Then, antimicrobial activity was evaluated by duel culture method and micro-dilution method. As a result, the water soluble extract of marine fungus Penicillium oxalicum 0312F1 had inhibitory activity against Alternaria solani. We also tested antitumor activity using methyl thiazolyl tetrazolium (MTT) method. The results showed that the fractions F gave the maximum inhibition rate (higher than 70%) against both human gastric cancer cell line (SGC-7901) (SGC-7901 cell line) and human hepatic cancer cell line (BEL-7404) (BEL-7404 cell line), at the concentration 0.5 mg/mL. While at the concentration of 1 mg/mL, the inhibition rates of fractions D, E, F against SGC-7901 cell line was higher than 70%, the inhibition rates of fractions C and F against BEL-7404 cell line was higher than 70%. There were antimicrobial and antitumor active fractions in secondary metabolites from marine fungus P. oxalicum 0312F1.

 

Key words: Antimicrobial, antitumor, crude secondary metabolites, Penicillium oxalicum.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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