African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

The discriminative power in determining genetic diversity of Escherichia coli isolates: Comparing ERIC-PCR with AFLP

Luka FAJS, Mateja JELEN, Maja BORIĆ, Tanja ĐAPA, Darja ŽGUR-BERTOK and Marjanca STARČIČ ERJAVEC*
Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, SI-1000 Ljubljana, Slovenia.
Email: [email protected]

  •  Accepted: 26 April 2013
  •  Published: 14 May 2013

Abstract

This present study was performed to investigate genetic diversity of Escherichia coliisolates from healthy cattle while comparing two methods namely, PCR assay for enterobacterial repetitive intergenic consensus sequences (ERIC-PCR) and amplified fragment length polymorphism (AFLP). Our study showed that ERIC-PCR is an appropriate/efficient method for determining diversity of E. coli, providing results comparable to those obtained by AFLP.

 

Key words: Escherichia coli, diversity, cattle, amplified fragment length polymorphism (AFLP), PCR assay for enterobacterial repetitive intergenic consensus sequences (ERIC-PCR).

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This article is published under the terms of the Creative Commons Attribution License 4.0

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