African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5093

Full Length Research Paper

Detection of mutations in ampC promoter/attenuator gene in Escherichia coli from dairy cows in Rio de Janeiro and Mato Grosso, Brazil

Gabrielli Stefaninni Santiago
  • Gabrielli Stefaninni Santiago
  • Department of Veterinary Microbiology and Immunology, Federal Rural University of Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, Brazil.
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Irene da Silva Coelho
  • Irene da Silva Coelho
  • Department of Veterinary Microbiology and Immunology, Federal Rural University of Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, Brazil.
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Beatriz Oliveira de Farias
  • Beatriz Oliveira de Farias
  • Department of Veterinary Microbiology and Immunology, Federal Rural University of Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, Brazil.
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Beatriz Oliveira de Farias
  • Beatriz Oliveira de Farias
  • Department of Veterinary Microbiology and Immunology, Federal Rural University of Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, Brazil.
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Tatiani Abreu de Alencar
  • Tatiani Abreu de Alencar
  • Department of Veterinary Microbiology and Immunology, Federal Rural University of Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, Brazil.
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Amy Borges Moreira
  • Amy Borges Moreira
  • Department of Infectious Diseases, Federal University of Mato Grosso, Alexander Ferronato Avenue, 1200, Sinop, MT, Brazil.
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Greiciane França Bronzato
  • Greiciane França Bronzato
  • Department of Veterinary Microbiology and Immunology, Federal Rural University of Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, Brazil.
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Miliane Moreira Soares de Souza
  • Miliane Moreira Soares de Souza
  • Department of Veterinary Microbiology and Immunology, Federal Rural University of Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, Brazil.
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Bruno Gomes de Castro
  • Bruno Gomes de Castro
  • Department of Infectious Diseases, Federal University of Mato Grosso, Alexander Ferronato Avenue, 1200, Sinop, MT, Brazil.
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Helena Neto Ferreira
  • Helena Neto Ferreira
  • Laboratory of Biological Science 18.P5.E3, Pharmacy Faculty of University of Porto, Jorge Viterbo Ferreira Street, 228, Porto, Portugal.
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Shana de Mattos de Oliveira Coelho
  • Shana de Mattos de Oliveira Coelho
  • Department of Veterinary Microbiology and Immunology, Federal Rural University of Rio de Janeiro, BR 465, Km 07, Seropédica, RJ, Brazil.
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  •  Received: 30 April 2019
  •  Accepted: 11 July 2019
  •  Published: 31 July 2019

Abstract

Escherichia coli present the ampC naturally, and the observation of phenotypical resistance to cefoxitin is related to this gene deregulation. Mutations in the regulatory region in ampC cause exaggerated expression. The most frequent alterations in the E. coli AmpC promoter/attenuator leading to this overexpression is described at the positions: -88, -82, -42, -18, -1 and +58. Mastitis studies were carried in Rio de Janeiro and Mato Grosso, Brazil. Two cefoxitin and amoxicillin-clavulanic acid-resistant E. coli from farms animals were unusually detected once these characteristics are not observed together in this species. The objective of this work was to determine if these isolates had a chromosomal gene mutation, determining AmpC hyperproduction. After DNA sequencing, mutations were observed at -88, -82, -73, -18, -1 and +58 positions, confirming the initially suspected AmpC hyperexpression. In Brazil, this is the first work to report E. coli hyperproducing this enzyme.

 

Key words: Ampc attenuator, AmpC hyperproduction, ampC promoter, bovine feces, mastitic milk.