African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5228

Full Length Research Paper

Application of the Alamar blue assay to determine the susceptibility to anti-tuberculosis pharmaceuticals

José de Jesús Alba-Romero1,2, José Luis Ortega-Sánchez5, Julieta Luna-Herrera3, Norma Urtiz-Estrada4, Maribel Cervantes-Flores4, Estela Ruíz-Baca4, José Ramón Hernández-Salgado5, Blanca Estela García-Pérez3, Pablo Ruíz-Flores6 and Aurora Martínez-Romero1*
  1Facultad de Ciencias Químicas, Unidad Gómez Palacio. Universidad Juárez del Estado de Durango (UJED). Av. Articulo 123 s/n Colonia Filadelfia CP 35010; AP No. 51, Gómez Palacio, Dgo., México. 2Laboratorio de Asesores Especializados de la Laguna. Juárez 142, Col. Centro. CP 35000. Gómez Palacio, Dgo., México. 3Departamento de Inmunología. Escuela Nacional de Ciencias Biológicas. Instituto Politécnico Nacional (IPN). Carpio y Plan de Ayala, Col. Santo Tomás 11340, México. 4Facultad de Ciencias Químicas, Unidad Durango. Universidad Juárez del Estado de Durango (UJED). Av. Veterinaria s/n, Circuito Universitario, Col. Valle del Sur. CP 34200. Durango, Dgo., México. 5Unidad Regional Universitaria de Zonas Áridas. Universidad Autónoma Chapingo.Bermejillo, Dgo., México. 6Departamento de Genética y Medicina Molecular. Centro de Investigación Biomédica. Universidad Autónoma de Coahuila (UA de C). Torreón, Coahuila., México.
Email: [email protected]

  •  Accepted: 29 September 2011
  •  Published: 16 November 2011

Abstract

 

The aim of this study was to apply the Alamar Blue assay to determine the susceptibility to anti-tuberculosis pharmaceuticals, in Mycobacterium tuberculosis isolated strains from patients with tuberculosis (TB). In this study, a TB diagnostic was done by bacilluscope as an essential diagnostic tool for pulmonary TB using the Ziehl-Neelsen (ZN) stain. Furthermore, a culture was prepared in Lowenstein-Jensen (L-J) solid media, Middlebrook 7H9, and Middlebrook 7H10. For all the clinical samples investigated, five reference strains of M. tuberculosis were used to standardize the sensibility to streptomycin (SM), isoniazid (INH), rifampicin (RIF), and ethambutol (EMB) of the ATCC. Additionally, 97Mycobacterium strains were analyzed from clinical isolations. The microplate Alamar Blue assay (MABA) was standardized and applied. The resistance levels of this disease were obtained in La Comarca Region (Lagunera). The results indicated that the MABA test is fast and easy to apply, however the most important aspect is the reliability of the method to determine the drug sensibility to pharmaceuticals. The MABA colorimetric test can be used in different regional as well as state TB diagnostic laboratories.

 

Key words: Tuberculosis, Alamar blue, drug resistance.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 5 No. 26
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES