African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Phytochemical screening of Azadirachta indica A. Juss for antimicrobial activity

Mariana C. Galeane*
  • Mariana C. Galeane*
  • São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, SP, Brazil. 2Franca University (UNIFRAN), Franca, SP, Brazil.
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Carlos H. G. Martins
  • Carlos H. G. Martins
  • São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, SP, Brazil. 2Franca University (UNIFRAN), Franca, SP, Brazil.
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Jaqueline Massuco
  • Jaqueline Massuco
  • São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, SP, Brazil. 2Franca University (UNIFRAN), Franca, SP, Brazil.
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Taís M. Bauab
  • Taís M. Bauab
  • São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, SP, Brazil. 2Franca University (UNIFRAN), Franca, SP, Brazil.
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Luís V. S. Sacramento
  • Luís V. S. Sacramento
  • São Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, SP, Brazil. 2Franca University (UNIFRAN), Franca, SP, Brazil.
  • Google Scholar


  •  Received: 13 October 2016
  •  Accepted: 24 November 2016
  •  Published: 28 January 2017

Abstract

Azadirachta indica A. Juss, known as neem (Meliacea family), has insecticide and pesticide properties, and many studies have shown their efficacy as antifungal, anti-inflammatory, among others. Studies for the development of drugs from plants are rising due to several factors such as bacterial resistance, indiscriminate use and the adverse reactions of antibiotics. In this study, phytochemical triage and thin layer chromatography analysis were performed, with similar results as the presence of flavonoids, tannins and terpenes. The antimicrobial activity showed that the ethyl acetate extract and butanol fraction presented greater activity against Streptococcus mutans and Streptococcus mitis presenting a MIC = 50 µg/ml for these strains, and the strain Enteroccocus faecalis, the hydroethanolic extract and aqueous fraction were most promising samples with a MIC = 50 µg/ml and MIC = 25 µg/ml, respectively. Therefore, it encourages the continuation of studies, aiming at the development of cosmetics or toothpaste.

Key words: Antimicrobial activity, Azadirachta indica, minimum inhibitory concentration, oral strains, phytochemistry.