Abstract

The antibacterial effects of Artemisia fragrans essential oil were studied in this research. The composition of essential oil from aerial parts was analyzed by GC/MS and its antibacterial effects were determined by disk diffusion method. In this study the antibacterial effects of essential oil of A. fragrans on two types of Gram positive bacteriaStaphylococcus aureus, Strep. Enterococcus fecalis and Gram negative bacteria;Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa and Klebsiella pneumonia by disk diffusion method on Mueller Hinton agar medium, were evaluated. The oil in concentrations 0, 10, 25, 50, 75, 100% prepared in DMSO and then 50 mg ml^-1 A. fragransessential oil was each was added to the specified disks. The present study describes the phytochemical profile and antibacterial activity of essential oil of A. fragrans. The antibacterial test results showed that the oil had a potential antibacterial activity against Gram positive bacterial strains that were mentioned. Essential oil showed maximum zone of inhibition concentration against Escherichia coli and Klebsiella pneumonia and minimal inhibition concentration against other Gram-negative bacteria that were studied.

Key words: Artemisia fragrans, antibacterial activity, essential oil, Gram negative bacteria, Gram positive bacteria, disk diffusion.