African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

An in-vitro model for studying the adhesion of Lactobacillus bulgaricus in soyghurt and enteropathogenic Escherichia coli (EPEC) on HEp-2 Cells

Jetty Nurhajati, Sayuti1*, Chrysanti2 and Syachroni1
  1Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University, Indonesia. 2Microbiology Department, Faculty of Medicine, Padjadjaran University, Indonesia.
Email: [email protected]

  •  Accepted: 15 February 2012
  •  Published: 28 June 2012

Abstract

 

Attachment or adhesion of Lactobacillus bulgaricus in soyghurt and enteropathogenicEscherichia coli (EPEC) with HEp-2 cells has been done. This research was aimed atfinding out the adhesion ability of L. bulgaricus and EPEC on HEp-2 cells experimentally in vitro. The results showed that L. bulgaricus in soyghurt could carry out adherence in HEp-2 cells and the best adhesion activity of L. bulgaricus was after 3 h of contact with HEp-2 cells. The longer the incubation time of contact, the greater the adhesion of L. bulgaricus.The next results of research showed that EPEC could carry out adherence by expressinglocalized adherence (LA) pattern and attaching and effacing lesion (A/E) forming apedestal on HEp-2 cells. L. bulgaricus in soyghurt has adhesive characteristics so it can be expected to carry out adherence in gastrointestinal tract and could inhibit EPEC adhesion activity.

 

Key words: Enteropathogenic Escherichia coli (EPEC), Lactobacillus bulgaricus, culture cell, bacterial adhesion, soyghurt.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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