Full Length Research Paper
Abstract
A cold-adapted lipase (LIP-BA) of Burkholderia anthina NT15 was purified by using PEG1000/potassium phosphate based aqueous two-phase systems, chromatography separation on a DEAE-cellulose-32 column and a Sephadex G100 column. Molecular weight of LIP-BA was determined to be approximately 44.5 kDa by SDS-PAGE. The optimum temperature for the activity of Lipase-BA was found to be 30°C. The optimum pH for the activity was 9.5.LIP-BA activity could be inhibited by Ca2+, Fe2+, La3+, Mn2+, K+, Sr2+, Fe3+, Cu2+ and Zn2+ or increased by Na+ and Mg2+. LIP-BA was stable in the solvent of isopentanol, ether and n-butanol. Tween-40 and Tween-60 could enhance the enzyme activity. The Km, Vmax and Kcat values of LIP-BA were 0.10 mM, 430 U/mg and 210 S-1, respectively.
Key words: Lipase, Burkholderia anthina, purification, characterization.
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