Abstract

Pseudomonas aeruginosais is an opportunistic Gram negative microorganism, usually related to serious infections within hospital environment and causes significant increase in patient’s morbidity and mortality. This study aimed to report antibiotic susceptibility of P. aeruginosa originated from all kind of specimens received at Clinical Microbiology Laboratory of the Faculty of Medicine, University of Indonesia, Jakarta, from 2004 to 2010, and evaluate their trend of susceptibility to certain antibiotics. Culture and identification of specimens were performed according to standard microbiology procedures. Antibiotic susceptibility tests were carried out according to performance standards for antimicrobial susceptibility testing from the Clinical and Laboratory Standards Institute. The data was processed using WHO-NET Version 5.6 program. P. aeruginosa was constantly found between 12 and 19% among other Gram negative bacteria. A significant decrease in susceptibility against ceftazidim, cefepime, cefoperazone, gentamicin, amikacin, tobramycin, ciprofloxacin, levofloxacin, meropenem and imipenem was observed. Susceptibility to aztreonam and piperacilline-tazobactam was decrease, though it was not statistically significant. In 2010, among the anti-pseudomonas antibiotics, imipenem showed good activity (80%). Overall, declining trend of susceptibility to all antibiotic tested was significantly observed. Imipenem was found to be the only anti-pseudomonas antibiotic with good activity (80%).

Key words: Gram negative bacteria, P. aeruginosa, antibiotic susceptibility.