African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Biological control of citrus green mould, Penicillium digitatum, by antifungal activities of Streptomyces isolates from agricultural soils

Shojaee Najmeh
  • Shojaee Najmeh
  • Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, Iran
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Shahidi Bonjar Gholam Hosein
  • Shahidi Bonjar Gholam Hosein
  • Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, Iran
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Shahdaei Sareh*
  • Shahdaei Sareh*
  • Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, Iran
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Leyla Shahidi Bonjar
  • Leyla Shahidi Bonjar
  • Department of Pharmacology, College of Pharmacy, Kerman University of Medical Sciences, International Campus, Kerman, Iran
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  •  Received: 19 August 2013
  •  Accepted: 21 February 2014
  •  Published: 02 April 2014

Abstract

Streptomyces strains can control postharvest citrus fruit diseases caused by Penicillium digitatum. One hundred and ten (110) Streptomyces strains were isolated from agricultural soils and screened against P. digitatum. The antagonistic activities of the isolates were determined through dual culture technique. In the 110 isolates, only strain 328 showed strong antifungal inhibitory effects. Streptomyces strain 328 showed a maximum biomass value (1.85 g/L) after 72 h incubation in yeast extract, malt extract, glucose (YMG) medium; in starch casein medium, the highest value was 0.68 g/L after 96 h incubation. The metabolites produced in starch casein medium showed the maximum inhibition zone against P. digitatum (28 mm diameter). Streptomyces strain 328 metabolites have molecular mass higher than 2000 and they have fungistatic mode of action against P. digitatum. In in vivo assays, the selected isolate has significantly reduced severity of green mold on crops which have been stored under 24°C for 28 days as compared to the controls. The inhibition effect of about 95% was shown by strain 328.

 

Key words: Penicillium digitatum, Streptomyces spp., Actinomycetes, biocontrol.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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