African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

High prevalence of extended spectrum beta-lactamase (ESBL) producers in fatal cases of pediatric septicemia among the Enterobacteriaceae in the pediatric hospital of Annaba, Algeria

Asma Labid
  • Asma Labid
  • Unité de recherche sur les maladies infectieuses et tropicales émergentes, URMITE CNRS-IRD, UMR 7278, IHU Méditerranée infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France. Faculté des Sciences, département de biochimie, université Badji Mokhtar Annaba, BP 12 2300 Annaba, Algeria.
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Djamila Gacemi-Kirane
  • Djamila Gacemi-Kirane
  • Faculté des Sciences, département de biochimie, université Badji Mokhtar Annaba, BP 12 2300 Annaba, Algeria.
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Mohammed Timinouni
  • Mohammed Timinouni
  • Institut Pasteur, laboratoire de Bactériologie Moléculaire, Casablanca, 20360, Maroc.
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Kamel Amoura
  • Kamel Amoura
  • Laboratoire Central de Microbiologie, CHU Annaba, Algeria.
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Jean-Marc Rolain
  • Jean-Marc Rolain
  • Unité de recherche sur les maladies infectieuses et tropicales émergentes, URMITE CNRS-IRD, UMR 7278, IHU Méditerranée infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
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  •  Received: 19 August 2013
  •  Accepted: 05 February 2014
  •  Published: 26 February 2014

Abstract

The aim of this study was to determine the prevalence of extended spectrum beta-lactamase (ESBL) producers and to perform molecular typing of ESBL-encoding genes in Enterobacteriaceae from clinical isolates recovered from blood samples of children in Annaba, Algeria. A total of 42 clinical isolates were collected from March 2010 to July 2011 from the pediatric hospital of Annaba. The strains were identified by phenotypic tests and the ESBL-encoding genes was accomplished by PCR amplification and sequencing. Among these isolates, 10 ESBL isolates had CTX-M-15, 13 had TEM-1, one isolate had TEM-136, 2 were positive for SHV-11, 8 had SHV-12, 3 had SHV-28, 1 isolate contained SHV-32, and 1 isolate had SHV-133. A total of 45.2% of the patients died. The high prevalence of ESBL producers among Enterobacteriaceae, along with the observation of 19 fatal cases, is worrisome; therefore, we believe that national surveillance of antibiotic resistance should be urgently implemented in Algeria.

 

Key words: Antibiotic resistance, Enterobacteriaceae, Algeria, septicemia

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This article is published under the terms of the Creative Commons Attribution License 4.0

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