African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Identification of antifungal compound produced by Bacillus subtilis LB5 with ability to control anthracnose disease caused by Colletotrichum gloeosporioides

On-Uma Ruangwong1, Chi-I Chang2, Senghor Amadou Lamine3 and Wen-Jinn Liang4*  
1Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung, 91201, R.O.C. Taiwan. 2Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, 91201, R.O.C. Taiwan. 3Ministry of Agriculture Senegal, Crop Protection Service Dakar, Dakar-Senegal. 4Department of Plant Medicine, National Pingtung University of Science and Technology, Pingtung, 91201, R.O.C. Taiwan
Email: [email protected]

  •  Accepted: 20 March 2012
  •  Published: 30 April 2012

Abstract

Bacillus subtilis strain LB5 showed inhibitory activity on conidial germination ofColletotrichum gloesporioides causal agent of mango and wax apple anthracnose disease. The antifungal metabolites produced by B. subtilis strain LB5 were highly stable to heat, pH ranging from 3 to 11, and ultraviolet (UV) light. Cell-free supernatant of LB5 culture broth was extracted by using n-butanol to give the n-butanol soluble fraction, which was purified by reversed phase high-performance liquid chromatography (RP-HPLC) to obtain the major antifungal compound. The structure of antifungal compound was identified as iturin A-2, a cyclic lipopeptide antibiotic, based on the analysis of 1H and 13C nuclear magnetic resonance (NMR) and fast atom bombardment mass spectrometry (FAB-MS) spectra.

 

Key words: Bacillus subtilis LB5, Colletotrichum gloeosporioides, reversed phase high-performance liquid chromatography (RP-HPLC), nuclear magnetic resonance (NMR), fast atom bombardment mass spectrometry (FAB-MS), antifungal compound, iturin A-2.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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