African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5228

Full Length Research Paper

First report of Alternaria astragali as a causative agent of leaf spot disease in Polygonum multiflorum Thunb.

Guoru Xiong1#, Gengfeng Zhao2#, Li Li2 and Shuzhen Zhang1* # Authors contributed equally to this work  
1Institue of Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences, HaiKou 571101, China. 2Department of Biochemistry, Hainan Medical College, HaiKou 571101, China
Email: [email protected]

  •  Accepted: 10 January 2012
  •  Published: 23 January 2012

Abstract

Leaf necrosis was observed on leaves of Polygonum multiflorum Thunb. in Kunming, China, from 2008 to 2009. The causal fungus was isolated and pure cultured from the leaf spot lesions on prostate-specific antigen (PSA). Koch’s postulates were fulfilled by inoculating leaves in vitro in a moist chamber and in vivo in field. Morphological and internal transcribed spacer (ITS) region sequence analysis (FJ379591) revealed that the fungal strain shared 99% nucleotide identity with Alternaria astragali (EF110523). This is the first report of Aastragali causing leaf spot on P. multiflorum Thunb. in China.

Key words: Alternaria astragali, identification, internal transcribed spacer (ITS) analysis, leaf spot and Polygonum multiflorum Thunb