Full Length Research Paper
Abstract
Leaf necrosis was observed on leaves of Polygonum multiflorum Thunb. in Kunming, China, from 2008 to 2009. The causal fungus was isolated and pure cultured from the leaf spot lesions on prostate-specific antigen (PSA). Koch’s postulates were fulfilled by inoculating leaves in vitro in a moist chamber and in vivo in field. Morphological and internal transcribed spacer (ITS) region sequence analysis (FJ379591) revealed that the fungal strain shared 99% nucleotide identity with Alternaria astragali (EF110523). This is the first report of A. astragali causing leaf spot on P. multiflorum Thunb. in China.
Key words: Alternaria astragali, identification, internal transcribed spacer (ITS) analysis, leaf spot and Polygonum multiflorum Thunb
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