African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Identification of a chitinase from Apocheima cinerarius nucleopolyhedrovirus

Qinghua Wang
  • Qinghua Wang
  • The Key Laboratory of Forest Protection, State Forestry Administration of China, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China
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Yuzhu Wang
  • Yuzhu Wang
  • The Key Laboratory of Forest Protection, State Forestry Administration of China, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China
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Guoli Zhong
  • Guoli Zhong
  • Baoding Entry-exit Inspection and Quarantine Bureau, Hebei, China
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Yongan Zhang*
  • Yongan Zhang*
  • The Key Laboratory of Forest Protection, State Forestry Administration of China, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China
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  •  Received: 16 April 2015
  •  Accepted: 08 June 2015
  •  Published: 10 June 2015

Abstract

Baculoviruses are important biological control agents against insect pests. In this work, a chitinase from Apocheima cinerarius nucleopolyhedrovirus (ApciNPV) as a fusion protein was highly over-expressed in Escherichia coli M 15 strain and affinity purified on Ni Sepharose 6 Fast Flow column. The modeling structure of ApciNPV chitinase indicated the protein contains a polycystic kidney 1(PKD1) domain on N-terminal and a characteristic catalytic domain formed α/β triosephosphate isomerase (TIM) barrel fold like other family 18 glycohydrolases. The ApciNPV chitinase displayed both endo- and exo-chitinase activities using fluorescent oligosaccharides. Moreover, assessed by LC50 (50% lethal concentration) values, the ApciNPV chitinase showed insecticidal activity against Apocheima cinerarius, Spodoptera exigua, Hyphantria cunea, Helicoverpa armigera and Lymantria dispar. The results suggest that ApciNPV chitinase may offer a potential application as a new tool to control pest.

Key words: Apocheima cinerarius nucleopolyhedrovirus, chitinase, recombinant protein, insecticidal activity.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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