African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Inoculation of Bradyrhizobium with cellular additives and micronutrients in soybean seeds cultivated in Oxisol under no-tillage system

Leandro Rampim*
  • Leandro Rampim*
  • State University of West Parana, Unioeste, CCA/PPGA, Pernambuco Street No. 1777, P.O. Box 9, Zip Code 85960-000, city of Marechal Candido Rondon, Parana state, Brazil
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Maria Do Carmo Lana
  • Maria Do Carmo Lana
  • State University of West Parana, Unioeste, CCA/PPGA, Pernambuco Street No. 1777, P.O. Box 9, Zip Code 85960-000, city of Marechal Candido Rondon, Parana state, Brazil
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Paulo Ricardo Lima
  • Paulo Ricardo Lima
  • State University of West Parana, Unioeste, CCA/PPGA, Pernambuco Street No. 1777, P.O. Box 9, Zip Code 85960-000, city of Marechal Candido Rondon, Parana state, Brazil
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Jean Sergio Rosset
  • Jean Sergio Rosset
  • State University of Mato Grosso do Sul, UEMS, BR 163, km 20,2, Zip Code: 79980-000, city of Mundo Novo, Mato Grosso do Sul State, Brazil
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Jeferson Klein
  • Jeferson Klein
  • Pontifical Catholic University, PUC, city of Toledo, Parana state, Brazil
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Alfredo Richart
  • Alfredo Richart
  • Pontifical Catholic University, PUC, city of Toledo, Parana state, Brazil
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Vandeir Francisco Guimaraes
  • Vandeir Francisco Guimaraes
  • State University of West Parana, Unioeste, CCA/PPGA, Pernambuco Street No. 1777, P.O. Box 9, Zip Code 85960-000, city of Marechal Candido Rondon, Parana state, Brazil
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Marcos Vinicius Mansano Sarto
  • Marcos Vinicius Mansano Sarto
  • State University Paulista Julio de Mesquita Filho, UNESP, Lageado Farm, Alcides Soares Highway, Km 3, Zip Code 18610-300, city of Botucatu, Sao Paulo state, Brazil
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Jucenei Fernando Frandoloso
  • Jucenei Fernando Frandoloso
  • State University of West Parana, Unioeste, CCA/PPGA, Pernambuco Street No. 1777, P.O. Box 9, Zip Code 85960-000, city of Marechal Candido Rondon, Parana state, Brazil
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Danielle Mattei
  • Danielle Mattei
  • State University of Mato Grosso do Sul, UEMS, BR 163, km 20,2, Zip Code: 79980-000, city of Mundo Novo, Mato Grosso do Sul State, Brazil
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Paulo Vitor Dal Molin
  • Paulo Vitor Dal Molin
  • State University of West Parana, Unioeste, CCA/PPGA, Pernambuco Street No. 1777, P.O. Box 9, Zip Code 85960-000, city of Marechal Candido Rondon, Parana state, Brazil
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Jessica Koppo
  • Jessica Koppo
  • State University of West Parana, Unioeste, CCA/PPGA, Pernambuco Street No. 1777, P.O. Box 9, Zip Code 85960-000, city of Marechal Candido Rondon, Parana state, Brazil
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Paulo Sergio Diel
  • Paulo Sergio Diel
  • State University of West Parana, Unioeste, CCA/PPGA, Pernambuco Street No. 1777, P.O. Box 9, Zip Code 85960-000, city of Marechal Candido Rondon, Parana state, Brazil
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Aline Kelly Pomini de Souza
  • Aline Kelly Pomini de Souza
  • State University of West Parana, Unioeste, CCA/PPGA, Pernambuco Street No. 1777, P.O. Box 9, Zip Code 85960-000, city of Marechal Candido Rondon, Parana state, Brazil
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  •  Received: 27 July 2014
  •  Accepted: 02 February 2015
  •  Published: 17 June 2015

Abstract

In the bid to get increased yield of soybean, several studies on biological fixation of nitrogen have been done in order to enhance the results of this technology with cellular additives and micronutrients. The aim of this study was to evaluate the use of inoculant (Bradyrhizobium japonicum SEMIA and 5019) associated with cellular additives, cobalt and molybdenum (Fertiactyl® leg) in soybeans seeds in Oxisol cultivated under no-tillage system. The experiment was conducted in a randomized block with two treatments (with and without commercial product) and 10 repetitions, totaling 20 experimental plots in the cultivation of soybean. The experiment was done in 2011/2012 and 2012/2013. We evaluated the N content in grain yield and weight of 100 grain in the 2011/2012 and grain yield, weight of 100 grain and foliar content of N, Ca, Mg, K, P and S in the 2012/2013 crop. Inoculation of the B. japonicum with cellular additives, cobalt and molybdenum shows greater accumulation of N in soybean grain, but does not influence the weight of 100 grains and yield in the culture of soybean in 2011/2012 and 2012/2013. There was an increase of Cu and reduction of K, P and S in the leaf tissue of soybeans with the presence of the B. japonicum with cellular additives, cobalt and molybdenum (Fertiactyl® leg). It is recommended that farmers should not sow soybean with seed treated with fungicide and insecticide for 12 h together with B. japonicum inoculation, cellular additives, Co and Mo; they cause nutritional changes without interfering in the yield under no-tillage in Oxisoil.

Key words: Bradyrhizobium japonicum, Glycine max, seed treatment, glifosate, fungicide, insecticide.