African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5228

Full Length Research Paper

Isolation and characterization of rhamnolipid producing Pseudomonas aeruginosa strains from waste edible oils

Xiangsheng Zhang1,2*, Dejun Xu1, Guocui Yang1, Huabin Zhang1, Junhui Li2 and Hojae Shim2
1Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, College of Life Science and Engineering, Yancheng Teachers University, Yancheng 224002, China. 2Department of Civil and Environmental Engineering, Faculty of Science and Technology,University of Macau, Macau SAR, China.
Email: [email protected]

  •  Accepted: 15 December 2011
  •  Published: 23 February 2012

Abstract

This study was to screen efficient biosurfactant producing strains from waste edible oils (WEOs). Two strains Y5 and Y17 were screened out by steps of preliminary blood plate screening and secondary flask fermentation screening. The surface tension of the fermentation broth of the two strains diluted by 100 times was lower than 40 mN/m. Preliminary characterization of fermentation products was conducted, which was found to be rhamnolipid ultimately. Y5 strain produced only one kind of glycolipids while the Y17 produced at least three kinds of homologues of glycolipids. Both Y5 and Y17 were identified as Pseudomonas aeruginosa strains. This study gave a simple and efficient protocol to screen out biosurfactant producing strains and would pave the way for the production of biosurfactant from WEOs.

Key words: Rhamnolipid, biosurfactant, fermentation, waste edible oils, Pseudomonas aeruginosa.

Abbreviation

ANOVA, Analysis of variance; BP, beef-peptone medium; FT-IR, Fourier transform infrared spectrum; NA, nutrient agar medium; TLC, thin-layer chromatography; WEOs, waste edible oils.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 6 No. 7
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES