African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Short Communication

Isolation and molecular detection of Plesiomonas shigelloides containing tetA gene from Asian arowana (Scleropages formosus) in a Korean aquarium

Jin Woo Jun1, Ji Hyung Kim1, Casiano H. Choresca Jr.1, Sang Phil Shin1, Jee Eun Han1, Dal Sang Jeong2 and Se Chang Park1*
  1Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea. 2Department of Aquaculture, Korea National College of Agriculture and Fisheries, Hwaseong 445-760, Republic of Korea.
Email: [email protected]

  •  Accepted: 09 November 2011
  •  Published: 30 November 2011

Abstract

 

An Asian arowana (dragonfish; Scleropages formosus) that had been reared for public exhibition in a private commercial aquarium in Seoul Korea was recently found dead. A bacterial pathogen was cultured from the fish’s kidney, identified, and confirmed asPlesiomonas shigelloides, using a Vitek System®2, PCR and 16S rRNA gene sequencing. The isolated strain proved resistant to commercial antibiotics and the presence of the tetracycline resistance gene (tetA) was detected via genetic analysis. In this paper, we have described the isolation and identification of P. shigelloides from an Asian arowana reared in a private aquarium in Korea.

 

Key words: Asian arowana (dragonfish; Scleropages formosus), Plesiomonas shigelloides, tetracycline resistance gene (tetA).