Abstract

Present investigation was the first attempt which deals with the in vivo and in vitrocomparative study of protein level in Acacia nilotica L., a nitrogen fixing tree. Protein was investigated in callus, seed, leaf and stem by means of SDS-PAGE. For obtaining the in vitro explant, the seeds were cultured on ½ MS medium under the in vitro condition. The cotyledonary nodal explants were taken from the in vitro seedlings and cultured in the MS medium supplemented in combination of 2, 4-D (0.4 mg/l) with BAP (0.25 mg/l). Though some differences were observed in the protein contents of in vivo and in vitro samples, the data proved that protein content in callus was higher than the seed following leaf and stem. In this study it was found that A. nilotica contained several protein bands of molecular weight 54.3, 44.1, 42.7, 40.1, 35.6, 31.2, 28.6, 24.7 and 19.5 kDa. These results indicate that the intensity of protein bands was high in in vitro sample compared to in vivosamples.

Key words: SDS-PAGE, in vitro, in vivo