Antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from bovine subclinical mastitis

Cristina Hallal de Freitas; Carolina Lambrecht GonÃ§alves; Josiara Furtado Mendes; Deborah Murowaniecki Otero; ClÃ¡udio Martin Pereira de Pereira; Helenice de Lima Gonzales; PatrÃ­cia da Silva Nascente

doi:10.5897/AJMR2016.8024

African Journal of
Microbiology Research

Abbreviation: Afr. J. Microbiol. Res.
Language: English
ISSN: 1996-0808
DOI: 10.5897/AJMR
Start Year: 2007
Published Articles: 5233

Full Length Research Paper

Antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from bovine subclinical mastitis

Cristina Hallal de Freitas

Cristina Hallal de Freitas
Programa de PÃ³s-GraduaÃ§Ã£o em BioquÃmica e BioprospecÃ§Ã£o, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
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Carolina Lambrecht GonÃ§alves

Carolina Lambrecht GonÃ§alves
Programa de PÃ³s-GraduaÃ§Ã£o em Parasitologia, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
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Josiara Furtado Mendes

Josiara Furtado Mendes
Programa de PÃ³s-GraduaÃ§Ã£o em VeterinÃ¡ria, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
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Deborah Murowaniecki Otero

Deborah Murowaniecki Otero
Instituto Federal Farroupilha, Campus Jaguari, RS, Brasil.
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ClÃ¡udio Martin Pereira de Pereira

ClÃ¡udio Martin Pereira de Pereira
Programa de PÃ³s-GraduaÃ§Ã£o em BioquÃmica e BioprospecÃ§Ã£o, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
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Helenice de Lima Gonzales

Helenice de Lima Gonzales
Faculdade de VeterinÃ¡ria LaboratÃ³rio de InspeÃ§Ã£o de Produtos de Origem Animal, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
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PatrÃcia da Silva Nascente

PatrÃcia da Silva Nascente
Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
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Article Number - 5D9C89559084
Vol.10(23), pp. 861-865 , June 2016
https://doi.org/10.5897/AJMR2016.8024

Received: 23 March 2016
Accepted: 22 April 2016
Published: 21 June 2016

Abstract

Concern with traces of antibiotics found in milk and resistant bacteria has led to the search for alternatives, mainly natural products, to treat mastitis. This study aimed at evaluating the antimicrobial activity of fixed oils found in Brazil nuts and sunflower seeds against microorganisms isolated from mastitic milk and at analyzing their fatty acids. The following microorganisms were isolated from milk produced by cows with subclinical mastitis and selected for the antimicrobial test: Staphylococcus aureus, Enterococcus spp., Candida spp. and Cryptococcus laurentii. The oil components and the antimicrobial activity were determined by gas chromatography and by the broth microdilution technique, respectively. The following fatty acids were identified: myristic, palmitic, palmitoleic, margaric, stearic, oleic, linoleic, linolenic, arachidic, gadoleic, behenic and lignoceric. Averages of minimum inhibitory concentrations (MIC) for S. aureus, Enterococcus spp., Candida spp. and C. laurenti were 36.30; 21.35; 15.62 and 10.41 μg.mL^-1 (Brazil nut oil) and 23.5; 19.21; 7.81 and 1.2 μg.mL^-1 (sunflower seed oil), respectively. These values show that these oils, in low concentration, have antimicrobial activity against the microorganisms under study. Therefore, this study shows that these fixed oils may be used as therapeutic resources for the control of mastitis.