Abstract

Studies were conducted to develop the sustainable plant protection strategies against leaf blight of green gram incited by Macrophomina phaseolina by using plant extracts and fungicides under in vitro and in vivo conditions. In vitro studies indicated that plant leaf extracts of Adenocalymma alliaceum (77.20%) and Allium sp. (74.71%) demonstrated the highest inhibition of mycelia growth of M. phaseolina at 10% concentration. The results revealed the significant performance by foliar spray of carbendazim (0.1%), mancozeb (0.2%) and leaf extracts of A. alliaceum (10%), Allium sp. (10%) was more effective in reducing leaf blight disease in mungbean plants under greenhouse conditions. Further, induction of defense enzymes, such as peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), phenols and also chlorophyll content were studied. The enzyme accumulation was greater in treated plants as compared to untreated control. The present study revealed the induced systemic resistance (ISR) in enhancing the disease resistance in green gram plants against leaf blight disease by the application of plant extracts and fungicides.

Key words: Macrophomina phaseolina, induced systemic resistance (ISR),Adenocalymma alliaceum, Allium sp.