African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Antifungal peptide produced by Paenibacillus polymyxa BRF-1 isolated from soybean rhizosphere

  Xueli Chen1, 2, Guanghua Wang1*, Meina Xu1, Jian Jin1 and Xiaobing Liu1  
  1Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, 150081, People’s Republic of China. 2Key Laboratory of Soil Environment and Plant Nutrition of Heilongjiang Province, Institute of Soil Fertility and Environmental Resources, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, People’s Republic of China.  
Email: [email protected]

  •  Accepted: 07 December 2010
  •  Published: 18 December 2010

Abstract

 

A bacterial strain BRF-1 that produced antifungal peptide was isolated from soybean rhizosphere and identified as Paenibacillus polymyxa by combination approaches of morphological and physiological tests, Biolog identification and 16S rDNA sequencing.Its cell free metabolite can inhabit many plant pathogenic fungi growth and its activity was stable to proteinase K digestion, autoclave and lower pH treatments. The antifungal substance was separated by (NH4)2SO4 and purified by Sephadex G-50, and acquired short peptide with molecular weight about 35.4 kDa, which has antifungal activity against Rhizoctonia solani.

 

Key words: Paenibacillus polymyxa, Biolog identification, 16S rDNA, antifungal activity, peptide.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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