African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Comparison of acid-fast bacilli smear and chromatographic immunoassay in patients with active tuberculosis

Aurora Martínez-Romero1*, José Luis Ortega-Sánchez5, María Guadalupe González-Yañez1,3, José Ramón Hernández-Salgado5, Estela Ruíz-Baca4, José de Jesús Alba-Romero1,2 and Maribel Cervantes-Flores4
1Facultad de Ciencias Químicas, Unidad Gómez Palacio. Universidad Juárez del Estado de Durango (UJED). Av. Articulo 123 s/n Colonia Filadelfia CP 35010; AP No. 51, Gómez Palacio, Dgo., México. 2Laboratorio de Asesores Especializados de la Laguna. Juárez 142, Col. Centro. CP 35000. Gómez Palacio, Dgo., México. 3Instituto de Seguridad y Servicio Social de los Trabajadores del Estado (ISSSTE).Hospital General C. Dr. Francisco Galindo Chavez. Torreón, Coahuila, México. 4Facultad de Ciencias Químicas, Unidad Durango. Universidad Juárez del Estado de Durango (UJED). Av. Veterinaria s/n, Circuito Universitario, Col. Valle del Sur. CP 34200. Durango, Dgo., México. 5Unidad Regional Universitaria de Zonas Áridas. Universidad Autónoma Chapingo.Bermejillo, Dgo., México
Email: [email protected]

  •  Accepted: 05 December 2011
  •  Published: 29 February 2012

Abstract

The aim of this study was to compare acid-fast bacilli smear and chromatographic immunoassay in patients with active tuberculosis (TB). The ability to rapidly detect tubercle bacilli in respiratory secretions was determined for the acid-fast bacilli (AFB) smear in comparison with chromatographic immunoassay in serum samples. A total of 96 respiratory specimens and 96 serum samples were evaluated. The chromatographic immunoassay and AFB were positive in six patients (6.25%) and negative in 90 of 96 specimens (93.75%). The sensitivity, specificity, positive predictive value and negative predictive value of chromatographic immunoassay and AFB were 100%. These results demonstrated the clinical usefulness of chromatographic immunoassay assay for the rapid diagnosis of TB in serum. And compared with the AFB smear, the chromatographic immunoassay assay gives a successful diagnosis.

 

Key words: Tuberculosis, chromatographic immunoassay, acid-fast bacilli smear.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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