African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Efficient cellulase production from low-cost substrates by Trichoderma reesei and its application on the enzymatic hydrolysis of corncob

Lian Xiong1, 2#, Chao Huang1,2#, Wan-feng Peng1, 2, Lv-rong Tang1, 2, Xiao-yan Yang1, 2, 3, Xue-fang Chen1, 2, 3, Xin-de Chen1, 2*, Long-long Ma1, 2 and Yong Chen1, 2
1Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China. 2Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People’s Republic of China. 3Graduate University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China.
Email: [email protected]

  •  Accepted: 10 May 2013
  •  Published: 25 October 2013


In this work, cellulase production by Trichoderma reesei CH11 using different low-cost raw materials as carbon sources and its application on the enzymatic hydrolysis of corncob were systematically investigated. Among all the studied carbon sources, rice straw was the most suitable one for T. reesei to produce cellulolytic enzymes. In addition, adding wheat bran and ammonium sulfate improved the cellulase activity and reduced the production cost. T. reesei was cultivated at 28°C and 160 rpm for five days to generate the liquid enzyme for hydrolysis. The enzyme activity of FPase reached 2.40 IU/mL, which was improved about 21% compared with that obtained from original culture medium without wheat bran or ammonium sulfate. Pretreated corncob was then hydrolyzed by this crude enzyme. After optimization of solid-to-liquid ratio and dilution rate of crude enzyme, 95.7% of hydrolysis yield was obtained. This work could offer one promising low-cost platform for bio-refinery.


Key words: Trichoderma reesei, cellulase, low-cost substrate, corncob, enzymatic hydrolysis.