African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5182

Full Length Research Paper

Genistein inhibits expression of matrix metalloproteinases-2 (MMP-2) in premature ovarian failure (POF) rats induced with cisplatin intraperitoneal injection

Hao Ai1*, Haiying Zhang2, Yun Zhang1 and Yang Liu1
1Third Affiliated Hospital of Liaoning University Medicine, Jinzhou, Liaoning 121001, China. 2Liaoning University of Medicine, Jinzhou, Liaoning 121001, China.
Email: [email protected]

  •  Accepted: 15 May 2012
  •  Published: 06 November 2012

Abstract

Matrix metalloproteinases (MMPs) play a pivotal role in ovary disease by degrading multiple elements of the extracellular matrix (ECM). Therefore, the inhibition of MMPs has been suggested to be a promising therapeutic strategy for premature ovarian failure(POF). Genistein, the primary isoflavone in legumes, has a well known weak estrogenic effect by binding to estrogen receptors, and widely used in the treatment of POF;however, MMPs changes after using genistein (Gen) was unclear. The aim of our study was to evaluate changes from MMP-2 of POF rats induced by cisplatin intraperitoneal injection after using different dose of GEN treatment by western-blot and immunohistochemistry technology. The results demonstrated that MMP was significantly down-regulated in middle dose GEN group and high dose of GEN group in comparison to the cisplatin group on protein level (P < 0.05), and middle dose GEN group, high dose of GEN group and control group had no significant difference on mRNA level (P < 0.05). The present study provides improvement in understanding the molecular pathogenic mechanism of POF and development of Gen as effective treatment drugs.

 

Key words: Genistein, premature ovarian failure, MMP-2, cisplatin, western-blot, immunohistochemistry.