African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Investigation of genetic variability among different isolates of Fusarium solani

Uzma Bashir*, Sidra Javed and Muhammad Shafiq
Institute of Agricultural Sciences, University of the Punjab, Quaid-e-Azam Campus Lahore, Pakistan.
Email: [email protected]

  •  Accepted: 26 March 2012
  •  Published: 28 June 2012

Abstract

 

Fusarium solani (Mart). Sacc. is an important wilt causing pathogenic fungi. Plant diseases have caused severe losses to human beings. In the present investigation five different isolates of F. solani were subjected to genetic variability analysis in terms of DNA-polymorphism using RAPD-PCR. F. solani isolate V3 (Lycopersicum esculentumMill.) and V5 (Solanum melongena L.) showed 78.2% similarity. Sequences of isolates V1(Lense esculentum L.) and V 2 (Acacia sp.) were 74.2% similar. Isolates (V1 and V2) and (V3 and V5) also show 65.5% similarities among their sequences. Whereas isolate V4(Gladiolus sp.) gives 70.3% similar results. Genetic variability pattern among isolates of F. solani were also supported by UPGMA dendrogram and percentage similarity table.

 

Key words: Acacia sp. Fusarium solani, Gladiolus sp. Lens esculenta, Lycopersicum esculentum, RAPD-PCR Solanum melongena.

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