African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Analysis of bacteria associated with Acropora solitaryensis by culture-dependent and -independent methods

Liu Z. H.1,2, Chen W.1,2, Gao L.1,2, Zhao JJ1,2, Ren C.H.1, Hu C. Q.1 and Chen C.1,2*
  1The Key Laboratory of Marine Bio-resources Sustainable Utilization(LMB), South China Sea Institution of Oceanology, Chinese Academy of Sciences，Guangzhou, 501301, China. 2The Ocean Observation and Research Station at XiSha Island, South China Sea Institution of Oceanology, Chinese Academy of Sciences, China. 3The graduate school of Chinese Academy of Sciences, Beijing, 10049, China.
Email: [email protected]

  •  Accepted: 23 February 2012
  •  Published: 28 June 2012

Abstract

 

Diversity of bacteria associated with Acropora solitaryensis, the main species in Hermatypic corals of XiSha Island, was investigated using culture-dependent and culture-independent methods (denature gradient gel electrophoresis, DGGE). It shows rich diversity of coral-associated bacteria with abundant novel species or genus. However, the diversity gained by the two methods was different. Among the bacteria identified by DGGE, XSLJ4 (Psychrobacter sp. KOPRI 25503), XSLJ6 (Rhizobium sp.), XSLJ11, (Uncultured Pseudomonadales), XSLJ12 (Ochrobactrum sp. Yw28) and XSLJ 13(Ochrobactrum sp. B10B）are the predominant species, while the vibrios were the predominant ones identified by bacterial culture method.

 

Key words: Acropora solitaryensis, diversity of bacterium denature gradient gel electrophoresis (DGGE)

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