Abstract

Three types of strains; CW-KD 4^T, MN28^T and DCY12^T which were published by Zhou et al. (2008) as Sinobacter flavus gen. nov., sp. nov., Friedrich et al. (2008) asSingularimonas variicoloris gen. nov., sp. nov., and Kim et al. (2007) as Solimonas soligen. nov., sp. nov., respectively, were selected and subjected to reclassification analyses using the polyphasic taxonomy. Phylogenetic analysis displayed that the three strains represented a deep-separated lineage within the family, Sinobacteraceae. The 16S rRNA gene sequence similarities among the three strains were 99.7% (CW-KD 4^T and MN28^T), 98.0% (CW-KD 4^T and DCY12^T) and 98.1 (MN28^T and DCY12^T). The isoprenoid quinone of the three strains was Q-8. The major fatty acids of strains CW-KD 4^T and MN28^T were C_16:0 and Sum in Feature 5, but the major fatty acids for strain DCY12^T were anteiso-C_15:0 and anteiso-C_17:0. The G+C contents of the DNA were 68.1 mol% (MN28^T), 65.8 mol% (CW-KD 4^T) and 63.5 mol% (DCY12^T). The co-predominant polar lipids of the three type strains were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. DNA–DNA hybridization results showed the levels of relatedness of 59.0% (MN28^T-DCY12^T), 21.4% (DCY12^T-CW-KD 4^T) and 37.5% (MN28^T-CW-KD 4^T) among each other. On basis of phenotypic and genotypic characteristics of the present study, the three type strains were reclassified as different species of the same genus Sinobacter, and Sinobacter variicoloris comb. nov and Sinobacter soli comb. nov. were proposed for the strains MN28^T and DCY12^T, respectively. The type strains are MN28^T (=DSM 15731^T=LMG 22844^T) and DCY12^T (=DSM 21787^T=LMG 24014^T =KCTC 12834^T).

Key words: Sinobacter, polyphasic taxonomy, type strain, reclassification.