African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Optimization studies on cellulase enzyme production by an isolated strain of Aspergillus niger YL128

  Mohammed Inuwa Ja’afaru1* and Obasola Ezekiel Fagade2  
  1Department of Microbiology, Federal University of Technology, P. M. B. 2076, Yola, Nigeria. 2Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria.  
Email: [email protected]

  •  Accepted: 24 September 2010
  •  Published: 18 December 2010

Abstract

 

Aspergillus niger YL128 was isolated from soil samples in Yola, Adamawa State, Nigeria. The organism produced cellulase enzyme optimally at 30ËšC. Growth of the organism at 50ËšC gave the least cellulase production. The highest amount of enzyme production was on the 9th day of incubation. Production of the cellulase enzyme was best at pH 5.0 and at carboxymethyl cellulase concentration of 1%. Enzyme synthesis was repressed in the presence of CuCl2, ZnCl2 and AlCl3, while the lignocellulosic wastes saw dust, wheat bran and sugarcane bagasse were good inducers. The isolate showed good ability of producing cellulase.

 

Key words: Aspergillus niger, cellulose, β-glucosidase, endoglucanase.