African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Purification and characterization of chitinase from Micrococcus sp.AG84 isolated from marine environment

  N. Annamalai*, S. Giji, M. Arumugam and T. Balasubramanian  
Centre of Advanced Study (CAS) in Marine Biology, Annamalai University, Parangipettai 608502, India.
Email: [email protected]

  •  Accepted: 07 December 2010
  •  Published: 18 December 2010

Abstract

 

Chitinase producing bacterial strain Micrococcus sp. AG84 isolated from marine sediments grew maximally in shake flask and produced chitinase at 35°C, pH 8.0. Chitinase activity was found to be maximum at 45°C, pH 8.0, and the enzyme was 100% stable even at 60°C and pH 11.0. Added with Fe2+, Ca2+ and Ni2+ chitinase activity increased but it was inhibited by EDTA. The molecular weight of purified chitinase is 33 kDa.

 

Key words: Micrococcus sp. AG84, chitinase, thermostable, alkaline, marine.

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