African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal

Ibou Diop*
  • Ibou Diop*
  • 1.Departement de Biologie Vegetale, Faculte des Sciences et Techniques, Universite Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Senegal; 2. IRD, Laboratoire Commun de Microbiologie (LCM/IRD/ISRA/UCAD), Bel-Air BP 1386, CP 18524, Dakar, Senegal.
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Fatou Ndoye
  • Fatou Ndoye
  • 1.Departement de Biologie Vegetale, Faculte des Sciences et Techniques, Universite Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Senegal; 2. IRD, Laboratoire Commun de Microbiologie (LCM/IRD/ISRA/UCAD), Bel-Air BP 1386, CP 18524, Dakar, Senegal.
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Aboubacry Kane
  • Aboubacry Kane
  • 1.Departement de Biologie Vegetale, Faculte des Sciences et Techniques, Universite Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Senegal; 2. IRD, Laboratoire Commun de Microbiologie (LCM/IRD/ISRA/UCAD), Bel-Air BP 1386, CP 18524, Dakar, Senegal.
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Tatiana Krasova-Wade
  • Tatiana Krasova-Wade
  • IRD, Laboratoire Commun de Microbiologie (LCM/IRD/ISRA/UCAD), Bel-Air BP 1386, CP 18524, Dakar, Senegal
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Alessandra Pontiroli
  • Alessandra Pontiroli
  • CIRAD, Laboratoire des Symbioses Tropicales et Mediterraneennes (LSTM), TA A-82 / J, 34398 Montpellier Cedex 5, France
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Francis A Do Rego
  • Francis A Do Rego
  • IRD, Laboratoire Commun de Microbiologie (LCM/IRD/ISRA/UCAD), Bel-Air BP 1386, CP 18524, Dakar, Senegal
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Kandioura Noba
  • Kandioura Noba
  • Departement de Biologie Vegetale, Faculte des Sciences et Techniques, Universite Cheikh Anta Diop de Dakar, BP 5005, Dakar-Fann, Senegal
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Yves Prin
  • Yves Prin
  • CIRAD, Laboratoire des Symbioses Tropicales et Mediterraneennes (LSTM), TA A-82 / J, 34398 Montpellier Cedex 5, France
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  •  Received: 10 March 2015
  •  Accepted: 05 May 2015
  •  Published: 27 May 2015

Abstract

The objective of this study was to characterize the diversity of arbuscular mycorrhizal fungal (AMF) communities colonizing the roots of Vigna unguiculata (L.) plants cultivated in two different sites in Senegal. Roots of cowpea plants and soil samples were collected from two fields (Ngothie and Diokoul) in the rural community of Dya (Senegal). Microscopic observations of the stained roots indicated a high colonization rate in roots from Ngothie site as compared to those from Diokoul site. The partial small subunit of ribosomal DNA genes was amplified from the genomic DNA extracted from these roots by polymerase chain reaction (PCR) with the universal primer NS31 and a fungal-specific primer AML2. Nucleotide sequence analysis revealed that 22 sequences from Ngothie site and only four sequences from Diokoul site were close to those of known arbuscular mycorrhizal fungi. Also, 47.6% of the clones from Ngothie site and 89.47% from Diokoul site were not close to known AMF. A total of 15 operational taxonomic units (OUT) were identified. Phylogenetic analyses showed that these clones belonged to the genera Glomus, Sclerocystis, Rhizophagus, Scutellospora, Gigaspora, Racocetra, Acaulospora and Redeckera. The genus Glomus is the most represented with six OTU, representing 40% of all OTU.

Key words: Agriculture, Glomeromycota, Vigna unguiculata, diversity, soil origin. 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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