Abstract

The characterisation of the causal agents of postharvest diseases, as well as their pathogenicity in different mango cultivars of economic importance in Mexico was evaluated. In total, 37 fungi were isolated from diseased mango (Mangifera indica L.) of the cvs Ataulfo, Hayden, Manila, Peach, Haden and creole. For the morphological characterization, the isolates were seeded in potato dextrose agar media and described by 4 variables. For the molecular characterization, DNA extraction was performed using the commercial Kit. For amplification, primers ITS5 and ITS4 were used and phylogenetic tree was built using RaxML. Six species were identified, namely Aspergillus niger, Colletotrichum asianum, Lasiodiplodia theobromae, Neofusicoccum oculatum, Pestalotiopsis mangiferea and Talaromyces variabilis. The most abundant group was the genus Aspergillus, with an appearance frequency of 0.35. Phylogenetic analysis showed that C. asianum and P. mangiferae belong to the families Glomerellaceae and Pestalotiopsidaceae, respectively, whereas A. niger and T. variabilis belong to the family Trichocomaceae. The fungi L. theobromae and N. occulatum belong to the family Botryosphaeriaceae. The pathogenicity of all isolates was demonstrated, except for T. variabilis. In contrast, L. theobromae and N. oculatum, were the most pathogenic isolates in all evaluated cultivars. Susceptibility to each pathogen differed among the cultivars, and Creole was most susceptible to the fungi evaluated.

Key words: Cultivars, disease postharvest, Mangifera indica L., phylogenetics analysis.