African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Efficacy of dichlorvos+tetramethrin, Bougainvillea glabra, potassium chloride and Bacillus thuringiensis on Tribolium castaneum

Kausar Malik*, Shaffaf Nizzami, Shagufta Andleeb, Amjad Farooq, Farkhanda Jabeen and Mir Muhammad Ali Talpur
Department of the Zoology, Lahore College for Women University, Lahore, Pakistan.
Email: [email protected]

  •  Accepted: 19 November 2012
  •  Published: 18 December 2012

Abstract

The present research was based on determining the effect of dichlorvos + tetramethrin (3.1% emulsifiable concentrate, EC), Bougainvillea glabra, potassium chloride and Shaf IBacillus thuringiensis alone or in combination against Tribolium castaneum (Jaculin Du val.) adults and larvae.  Bt was isolated from samples, cultured on different media by applying microbial techniques and then crystal protein was isolated from bacteria. The bio-toxicity was analyzsed by SPSS software and showed that larvae are more susceptible and showed significant results as compared to adults. Separate bioassays revealed that Shaf I B. thuringiensis was more toxic at all concentrations followed by an insecticide DDVP+tetramethrin, chemical potassium chloride and the least toxic was B. glabra. In the synergistic effect, highest mortality percentage was shown by the bioinsecticide, B. thuringiensis. All elements whenever combined together, proved to be useful for the control of stored product pests.

 

Key words: Dichlorvos (2-2, dichlorodivinyl dimethyl phosphate), Bacillus thuringiensis(Bt), EC), Statistical Package of Social Sciences (SPSS), T. castaneum.