African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Accumulation of some heavy metals by metal resistant avirulent Bacillus anthracis PS2010 isolated from Egypt

Sobhy E. Elsilk*
  • Sobhy E. Elsilk*
  • 1. Biological Sciences Department, Faculty of Science and Art, King Abdulaziz University, Rabigh Campus, P.O. Box: 344 Rabigh 21911, Saudi Arabia; 2. Bacteriology Unit, Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
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Abd El-Raheem R. El-Shanshoury
  • Abd El-Raheem R. El-Shanshoury
  • 2. Bacteriology Unit, Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt; 3. Biotechnology Department, Faculty of Science, Taif University, P.O. Box: 888, Taif 21974, Saudi Arabia
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Perihan S. Ateya
  • Perihan S. Ateya
  • Bacteriology Unit, Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
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  •  Received: 05 December 2013
  •  Accepted: 07 February 2014
  •  Published: 19 March 2014

Abstract

The bacteria with a high growth rate were isolated from polluted industrial waste water. The bacteria Bacillus anthracis PS2010 have variable resistant to heavy metals such as Cd, Cu, Co, Zn and Pb. Out of which the minimal inhibitory concentrations were 0.6, 2.0, 0.8, 4.0 and 3.0 mM, respectively. The potent bacterium has optimal biosorption capacity raised according to the metal, incubation temperature, pH of the solution and contact time. Under optimal conditions, the bacterium was capable of taking up the heavy metals Cd, Cu, Co, Zn and Pb at 3.41, 2.03, 4.75, 5.22 and 6.44 mg/g dry weight. Transmission electron microscopy showed accumulation of Pb metal external to bacterial cells. The mechanism of heavy metal tolerance in Bacillus anthracis PS2010 is chromosomally encoded. Bacillus anthracis harbored no plasmid.   

 

Key words: Heavy metal uptake, bacterial biosorption, plasmid, Bacillus anthracis PS2010.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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