African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5014

Full Length Research Paper

Naftifine inhibits pigmentation through down-regulation on expression of phytoene desaturase gene CAR1 in Rhodotorula mucilaginosa

Guowang Huang
  • Guowang Huang
  • Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
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Nur Fazleen Binti Idris
  • Nur Fazleen Binti Idris
  • Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
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Yimin Li
  • Yimin Li
  • Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
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Yang Wang
  • Yang Wang
  • Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
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Zeng Tu
  • Zeng Tu
  • Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
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  •  Received: 07 March 2020
  •  Accepted: 23 April 2020
  •  Published: 31 May 2020

Abstract

Naftifine, an antifungal drug, inhibits pigmentation in Rhodotorula mucilaginosa. However, the relative mechanism is minutely understood. In this study, regulation of gene expression by naftifine was investigated to elucidate mechanism of yeast de-pigmentation. RNA-sequencing (RNA-seq) was used to screen differentially expressed genes (DEGs), followed by quantitative PCR (qPCR). The qPCR results showed that mRNA expression of phytoene desaturase gene CAR1 was reduced to 37% of its original level, after one day’s naftifine treatment. Since CAR1 acts at the immediate upstream of carotenoid biosynthesis pathway, it was concluded that naftifine involves in the process to inhibit the activity of phytoene desaturase, and that the down-regulation of gene CAR1 by naftifine contributes to de-pigmentation in R. mucilaginosa.
 
Key word: Naftifine, carotenoid, Rhodotorula mucilaginosa, phytoene desaturase.